庚型肝炎病毒致病性的初步研究

目的 探讨庚型肝炎病毒（ＨＧＶ）的致病性。方法 应用ＲＴ－ｎＰＣＲ检测３６８例肝炎患者血清ＨＧＶ ＲＮＡ和血清酶的变化，并对其中１例单独庚型肝炎肝硬化病例进行肝脏活组织是检测。结果 在７１例急性黄疸型肝炎中检出单纯性ＨＧＶ ＲＮＡ阳性７例，１５５例慢性肝炎中检出单纯性ＨＧＶ ＲＮＡ阳性２２例，５１例肝硬化中检出单纯ＨＧＶ ＲＮＡ阳性３例。其中１例肝穿组织免疫组化证实为ＨＧＶ ＮＳ ５Ａｇ阳性。结论 争性黄疸型肝炎及乙、丙型肝炎病毒携带者，其慢性肝炎、肝硬化和肝癌中均可检出ＨＧＶ ＲＮＡ，ＨＧＶ感染可为单独感中与乙／丙型肝炎病毒混合或重叠感染，肝脏病理和免疫组化检查证实庚型肝炎病毒是一种嗜肝病毒，其定位主要存在于细胞浆内，可引起慢性病毒性肝炎甚至肝硬化。庚型肝炎病毒很可能具有致病性。

Preliminary study on pathogenicity of hepatitis G virus

OBJECTIVE: To study the clinical, enzymatic and liver pathological changes in patients with hepatitis G virus (HGV) infection. METHODS: RT- nested PCR was applied to detect HGV RNA in 368 patients with hepatic diseases. The liver biopsy tissue from one liver cirrhosis patient with simple HGV infection was examined. RESULTS: Simple HGV RNA positive were found in 7 cases of 71 acute hepatitis jaundice, in 22 cases of 155 chronic hepatitis and in 3 cases of 51 liver cirrhosis. The immunohistochemistry of the liver biopsy tissue from a liver cirrhosis patient showed HGV NS5 antigen positive. However, HGV infection might occur simply or in combination with hepatitis B or C virus or super infected. CONCLUSIONS: HGV RNA could be detected in acute hepatitis jaundice, chronic hepatitis, hepatitis B or C carriers, liver cirrhosis and hepatocellular carcinoma. HGV is proved to be a hepatotropic virus by pathological examination and immunohistochemistry, the virus located in the cytoplasm of the liver cells. It may cause chronic hepatitis and even liver cirrhosis, so HGV is pathogenic to liver tissue.