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| 地衣酸抑制前列腺癌PC-3M细胞增殖效应的初步探讨 | |
| 引用本文： | 孙 艳,王洪军,张 薇,吴 扬,张志强,冯 立,王立强,吴益民. 地衣酸抑制前列腺癌PC-3M细胞增殖效应的初步探讨[J]. 中国肿瘤生物治疗杂志, 2005, 12(4): 289-291 |
| 作者姓名： | 孙 艳 王洪军 张 薇 吴 扬 张志强 冯 立 王立强 吴益民 |
| 作者单位： | 1. 沈阳药科大学中药学院,沈阳,110016;沈阳军区疾病预防控制中心,沈阳,110034 2. 沈阳军区疾病预防控制中心,沈阳,110034 3. 东北师范大学,长春,130000 4. 吉林大学第一临床医院,长春,130021 |
| 基金项目： | 日本国际协力事业团资助项目 |
| 摘 要： | 目的:从真菌松萝中提取地衣酸并研究地衣酸对体外培养前列腺癌PC-3M细胞增殖的抑制效应.方法:用细胞培养、细胞生长抑制实验(MTT法)观察PC-3M细胞形态、细胞生长密度和检测细胞生长抑制率,用3H-TdR掺入法测定细胞增殖过程中DNA的含量.结果:与对照组相比,实验组PC-3M细胞形态异常、细胞生长密度降低,细胞生长抑制率增强,3H-TdR掺人量明显减少,并呈剂量依赖关系,与地衣酸浓度的相关系数分别为0.9799与-0.9525.结论:地衣酸对体外培养PC-3M细胞具有抑制效应. |
| 关 键 词： | 前列腺癌 地衣酸 |
| 文章编号： | 1007-385X(2005)04-0289-03 |
| 收稿时间： | 2005-09-10 |
| 修稿时间： | 2005-10-16 |
The Inhibitory Effect of Usnic Acid on Proliferation of the PC-3M Cells |
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| SUN Yan,WANG Hong-jun,ZHANG Wei,WU Yang,ZHANG Zhi-qiang,Feng Li,WANG Li-qiang and WU Yi-min. The Inhibitory Effect of Usnic Acid on Proliferation of the PC-3M Cells[J]. Chinses Journal of Cancer Biotherapy, 2005, 12(4): 289-291 | |
| Authors: | SUN Yan WANG Hong-jun ZHANG Wei WU Yang ZHANG Zhi-qiang Feng Li WANG Li-qiang WU Yi-min |
| Affiliation: | School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China; Centre of Disease Prevent and Control of Shenyang Command, Shenyang 110034, China;Centre of Disease Prevent and Control of Shenyang Command, Shenyang 110034, China;North China Normal University, Changchun 130000, China;Jilin University First Clinical Hospital, Changchun 130021, China;Centre of Disease Prevent and Control of Shenyang Command, Shenyang 110034, China;Centre of Disease Prevent and Control of Shenyang Command, Shenyang 110034, China;Centre of Disease Prevent and Control of Shenyang Command, Shenyang 110034, China;Centre of Disease Prevent and Control of Shenyang Command, Shenyang 110034, China |
| Abstract: | Objective: To extract Usnic acid from usnea longisima ach, and to evaluate the ability of Usnic acid to inhibit growth of human prostate cancer cells PC-3M in vitro. Methods: The cells were observed by microscope. The cytotoxicity to cancer cells was determined by MTT assay. DNA synthesis was tested by ~3H-TdR in corporation assay in the experiments. Results: There were significant differences between PC-3M cells lest group and control group. The Usnic acid had streng thened cytotoxicity, and the rate of ~3H-TdR incorporation was lower than that of the control in a dose-dependent manner (r =0.9799, r=-0.9525). Conclusion: The results suggested that the Usnic acid has inhibitory effects on growth of human PC-3M cells in vitro. |
| Keywords: | PC-3M |
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