Protective role of lycopene on cisplatin-induced changes in sperm characteristics, testicular damage and oxidative stress in rats |
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| Authors: | Ateşşahin Ahmet Karahan Izzet Türk Gaffari Gür Seyfettin Yilmaz Seval Ceribaşi Ali Osman |
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| Affiliation: | 1. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Fırat University, 23119 Elazığ, Turkey;2. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, 23119 Elazığ, Turkey;3. Department of Biochemistry, Faculty of Veterinary Medicine, Fırat University, 23119 Elazığ, Turkey;4. Department of Pathology, Faculty of Veterinary Medicine, Fırat University, 23119 Elazığ, Turkey;1. Department of Biochemistry, Faculty of Pharmacy, Cairo University, Egypt;2. Department of Pharmacology & Toxicology, Faculty of Pharmacy, Cairo University, Egypt;3. Department of Histology, Faculty of Medicine, Cairo University, Egypt;1. Institute of Toxicology, Shandong University, 44 West Wenhua Road, Jinan, Shandong, 250012, PR China;2. Center for Disease Control and Prevention of Hongta District, 34 Mingzhu Road, Yuxi, Yunnan, 653100, PR China;1. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Nahda University, Beni-Suef, 62514, Egypt;2. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514, Egypt;1. Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Bingol University Bingol, Turkey;2. Department of Biochemistry, Faculty of Veterinary Medicine, Bingol University, Bingol, Turkey;3. Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey;4. Department of Pathology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey;1. Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt, Medical Laboratory Department, Faculty of Applied Medical Science, Taif University, Taif, KSA;2. Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt;3. Histology and Cell Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt, Histology Department, Faculty of Oral Medicine and Dentistry, MTI University, Cairo, Egypt;4. Clinical Pharmacology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt |
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| Abstract: | The aim of this study was to investigate the possible protective role of lycopene on cisplatin (CP)-induced spermiotoxicity using quantitative, biochemical and histopathological approaches. Adult male Sprague-Dawley rats were randomly divided into four groups. The control group received physiological saline; animals in cisplatin group received only cisplatin; pre-treatment group received a 10-day of lycopene before administration of cisplatin while animals in post-treatment group received a 5-day of lycopene following administration of cisplatin. Cisplatin (7 mg kg(-1)) was intraperitoneally (i.p.) injected as a single dose and lycopene (4 mg kg(-1)) was administered by gavage in corn oil. Traits of reproductive organs; sperm characteristics, testicular histological findings, plasma testosterone levels and the testicular tissue oxidative status were determined. Administration of cisplatin to rats decreased sperm concentration (p < 0.05) and sperm motility (p < 0.001), increased total abnormal sperm rates (p < 0.05) as compared with the control group. While a marked normalization was achieved only in sperm concentration with lycopene in pre-treatment group, significant normalizations were achieved in the sperm concentration, sperm motility, total abnormal sperm rates in post-treatment group. No significant differences in levels of testosterone were observed among all groups. An increase in testes malondialdehyde concentrations (p < 0.05) and glutathione peroxidase activities (p < 0.001) were detected while significant decreases in glutathione levels (p < 0.001) in cisplatin alone group when compared to control group. While pre-treatment with lycopene restoring only malondialdehyde concentrations, its post-treatment caused normalization in both malondialdehyde and glutathione levels when compared with the cisplatin alone group. On the other hand, significant increases were determined in GSH-Px activities in all experimental groups when compared with the control group. Although the mechanism is not clear, the results from this experimental study suggest that the lycopene have a possible protective effect against cisplatin-induced spermiotoxicity, effect of giving lycopene after cisplatin being superior to the giving it before cisplatin. |
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