| 高效液相色谱法分析组合化学合成结构相似小分子多肽 |
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| 引用本文： | 董文玉,关福玉.高效液相色谱法分析组合化学合成结构相似小分子多肽[J].药物分析杂志,1999,19(4):222-224. |
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| 作者姓名： | 董文玉 关福玉 |
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| 作者单位： | 中国人民解放军总医院 |
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| 摘 要： | 目的：为组合化学合成结构相似小分子多肽提供快速准确的ＨＰＬＣ分析方法。方法：ＰｅｓｏｌｖＣ１８柱（５μｍ，４．６ｍｍ＊２００ｍｍ）。流动相：乙腈－水（２０：８０），内含０．１％三氟乙酸为流动相酸性调节剂。检测波长：２１４ｎｍ。结果：目标多肽纯度范围２６．３６％～７７．０２％，迁移时间２．８９～３０．１５ｍｉｎ。７号多肽纯度和迁移时间的ＲＳＤ分别为１．９％和１．４％。结论：应用本方法可快速准确分离分
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| 关 键 词： | 高效液相色谱法 组合化学 多肽 |
High Performance Liquid Chromatographic Analysis of StructurallySimilar Peptides by Combinatorial Chemistry Synthesis |
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| Dong Wenyu,Guan Fuyu,Liang Minglin and Xu Hong.High Performance Liquid Chromatographic Analysis of StructurallySimilar Peptides by Combinatorial Chemistry Synthesis[J].Chinese Journal of Pharmaceutical Analysis,1999,19(4):222-224. |
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| Authors: | Dong Wenyu Guan Fuyu Liang Minglin and Xu Hong |
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| Abstract: | Objective: To develop a HPLC method for assaying structurally similar peptides synthesized by combinatorial chemistry. Method : With Resolv C18 column(5 m,46 mm200 mm). Acetonitrile-H2O(2080) as mobile phase, 01% trifluoroacetic acid used as acidic modifier. Detector at 214 nm. Results: The purity of peptides ranges from 2636% to 7702%, migration time 2893015 min. The RSD of purity and migration time of sample 7 is 19% and 14% respectively. Conclusion:This method provides a simple and feasible way to analyze structurally similar peptides especially those synthesized by combinatorial chemistry. |
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| Keywords: | HPLC combinatorial chemistry peptides |
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