| 银杏内酯的快速测定方法的研究 |
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| 引用本文： | 姚渭溪,杨春.银杏内酯的快速测定方法的研究[J].药物分析杂志,1999,19(1):38-41. |
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| 作者姓名： | 姚渭溪 杨春 |
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| 作者单位： | 中国科学院生态环境研究中心 |
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| 基金项目： | 中国科学技术部生命科学发展中心资助 |
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| 摘 要： | 目的：介绍一种快速测定银杏内酯的新方法。方法：采用单柱层析和高效液相色谱联用的化分离技术。结果和结论：该方法可减少样品的预纯化时间，与目前的常规测定方法相比，其纯化时间缩短到常规方法的１／６，回收率高，相对标准偏差小，而且操作简便，易于掌握，可用于银杏叶及其提取物（ＧＢＥ）中银杏内酯的快速测定和质量监控。
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| 关 键 词： | 银杏内酯 白果内酯 高效液相色谱 PAF拮抗剂 |
Rapid Determination of Ginkgolides and Bilobalide in Leaves of Ginkgo biloba Lour and Its Extracts by HPLC |
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| Yao Weixi,Yang Chun,Tian Yu,Liu Tianmin and Xu Yanrong.Rapid Determination of Ginkgolides and Bilobalide in Leaves of Ginkgo biloba Lour and Its Extracts by HPLC[J].Chinese Journal of Pharmaceutical Analysis,1999,19(1):38-41. |
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| Authors: | Yao Weixi Yang Chun Tian Yu Liu Tianmin and Xu Yanrong |
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| Abstract: | Objective:This paper reported a rapid determining method of ginkgolide A,B,C and bilobalide in leaves of Ginkgo biloba Lour and its extracts.Method:The single chromatographic column as a purification column couple with HPLC system was used in the method.Results and Conclusion:The results showed that purification time has decreased to one sixths of the conventional method by using this method.The method was simple,reproducible,accurate and can be applied in rapid qualitative and quantitative determination of ginkgolides and bilobalide in leaves of Ginkgo biloba Lour and its extracts. |
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| Keywords: | Ginkgolides bilobalide purification chromatographic column high performance liquid chromatography refractive index detection |
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