首页 | 本学科首页   官方微博 | 高级检索  
     

喉部鳞状细胞癌组织中p_(53)蛋白的免疫转印分析
引用本文:邹静,尚耀东,彭子成,陶振峰,毕爱芳,高兰瑛. 喉部鳞状细胞癌组织中p_(53)蛋白的免疫转印分析[J]. 中国耳鼻咽喉头颈外科, 1997, 0(4)
作者姓名:邹静  尚耀东  彭子成  陶振峰  毕爱芳  高兰瑛
作者单位:石家庄白求恩国际和平医院耳鼻咽喉科!050082
摘    要:为观察喉部鳞状细胞癌及其癌旁组织中P53蛋白的降解及其寡聚物的形成,采用免疫转印法分析了15例喉部鳞状细胞癌标本中P53蛋白的电泳行为。发现P53蛋白阳性率在癌灶中心组织中为100%,在癌旁组织中为66.7%,两组间相差显著(P<0.05)。在所有标本均未发现P53蛋白寡聚物,且P53蛋白均有不同程度的降解,其中发生完全降解者(分子量均低于53kD)在癌灶中心组织中为60.0%,在癌旁组织中为30.0%(占阳性例数的比例),且癌旁组织中P53蛋白的平均分子量较癌灶中心组织低,但是,无统计学意义(P>0.05)。

关 键 词:喉肿瘤  癌.鳞状细胞  基因表达调节

Analysis of p_(53) protein in the tissues of laryngeal squamous cell carcinoma with Western blotting method
Zou Jing, Shang Yaodong, Peng Zicheng, Tao Zhenfeng, Bi Aifang, Gao Lanying. Analysis of p_(53) protein in the tissues of laryngeal squamous cell carcinoma with Western blotting method[J]. Chinese Archives of Otolaryngology-Head and Neck Surgery, 1997, 0(4)
Authors:Zou Jing   Shang Yaodong   Peng Zicheng   Tao Zhenfeng   Bi Aifang   Gao Lanying
Abstract:In order to investigate the hydrolysis or oligopolymerization of p53 protein in the tissues of laryngeal squamous cell carcinoma, wer have observed their electrophoretic action in 15 cases of the disease with Western blotting method. We found that the positive rate of p53 protein in the center carcinous tissues (100% ) was significantly higher than in the tissues adjacent to cancer (66. 7% ), (P<0. 05). No oligo-polymerization of p.3 protein was found in all the tissues, but hydrolyzed to a greater or lesser degree. Wholly hydrolytic (molecular weight of any bands below 53kD) rate was 60% in the center carcinous tissues and 30% in the adjacent tissues (of positive cases) respectively. The average molecular weight of p53 protein in the adjacent tissues was lower than in the center carcinous tissues, but not significantly (P>0.05). We speculate that during the canceration of laryngeal squamous cell carcinoma, mutated p53 protein mainly plays its cancerigenic role through acquire new function of overthrowing the negative regulating role of wild-type but not oligo-polymerization. Wild-type p53 protein may be hydorlyzed and lost negative regulating function. Mutated p53 protein was overexpressed in center carcinous tissues.
Keywords:
本文献已被 CNKI 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号