中国部分地区丁型肝炎的流行特点及特异性诊断试剂的制备研究

本研究采用免疫学方法对我国11个省、市自治区1027例乙型肝炎患者血清进行检测,抗-HD阳性率为1.7%。首次在我国新疆、内蒙和西藏等少数民族地区发现 HDV 感染,并对其与 HBsAg 亚型的关系进行了探讨。阐明了我国丁型肝炎的一些流行特征。采用 HDV 感染携带 WHV 的东方土拨鼠,建立了HDV 感染的实验动物模型。利用土拨鼠肝脏提取纯化 HDAg,制备了检测抗-HD 的诊断试剂盒。该试剂与爱尔兰 Noctech 公司同类试剂相比较,特异性和敏感性均相一致。重复性好,操作方便.诊断快速。可解决国内丁肝试剂短缺的问题。

Investigation of Epidemic Features of Hepatitis D in some areas of China and Preparation of specific Diagnostic Reagents

The prevelance of hepatitis delta virus(HDV)was evaluated in 1027 HBsAg positive patients living in 11 various areas of China.The positive rate of anti-HD was I.7 %.HDV infection was found in Xinjiang,Inner Mongolia and Tibet at the first time.Some epidemic features of Hepatitis D and its relation to HBsAg subtypes were discussed.We established experimental animal model for HDV infection by inoculating WHV carrier woodchucks with HDV.HDAg was separated from wood- chuck livers and diagnostic reagents kit for detecting anti-HD was prepared.Comparing with the kit of Noctech Limited Company,Ireland,specificity and sensitivity were identical in the two kind kits. Re- peated results were unified.Procedures of the kit simple and convenient.