IL-1B基因多态性和H.pylori感染与汉族人胃癌的相关性研究

目的：通过比较胃癌患者与匹配人群的幽门螺旋杆菌（Helicobacter pylori，H.pylori）感染率和IL-1B基因多态性，探讨IL-1B基因多态性是否增加H．pylori感染后胃癌发生的危险性。方法：采用聚合酶链反应-限制性片段长度多态性（Restriction fragment length polymorphism，RFLP）分析法检测胃癌低发区84例胃癌患者和84例与之性别、年龄匹配的普通人群的1L-1B基因多态性。采用酶联免疫吸附法（Enzyme—linked immunosorbent assay，ELISA）检测上述人群中的H.pylori感染率。结果：①胃癌患者IL-1B-511T／T基因型频率显著高于性别、年龄匹配的对照人群（P〈0．05），IL-1B-31T／T基因型频率在两组间无显著差异（P〉0．05）。84例胃癌患者的H.pylori感染率显著高于对照人群（P〈0．01）。胃癌患者H.pylori阳性感染者IL-1B-511 T／T基因型个体显著多于对照人群。结论：H.pylori感染者胃癌组织中IL-1B-511 T／T基因型为主，提示IL-1B-511 T／T基因型可能增加H.pylori感染后中国汉族人群发生胃癌的危险性，而IL-1B-31基因型与H.pylori感染后中国汉族人群胃癌发生无显著相关性。

Association of IL-1B gene polymorphism and H. pylori infection with gastric cancer in Chinese patients with Han nationality

Objective:The H.pylori infection rate of and IL-1B gene polymorphism in gastric cancer patients and normal people were investigated.The effect of IL-1B gene polymorphism on the development of gastric cancer inducing the H.pylori infection.Methods:IL-1B polymorphisms were analyzed with PCR-RFLP in 84 cases of gastric cancer and 84 cases of normal people in low-prevalence region. The H.pylori infection rates were examined with ELISA.Results:T/T carrier frequencies of IL-1B-511 was remarkably higher in gastric cancer patients compared with the normal people(P<0.05); T/T carrier frequencies of IL-1B-31 was similar in all of the population. H.pylori infection rate of 84 cases gastric cancer patients was remarkably higher than normal people(P<0.01). T/T carrier frequencies of IL-1B-511 was remarkably higher in gastric cancer patients H.pylori infected compared with the normal people.Conclusion:IL-1B-511 T/T genotype might be increase the risk of gastric cancer infected by H.pylori in Chinese population. IL-1B-31 genotype do not increases the risk of gastric cancer infected by H.pylori.