INTRATHYMIC INOCULATION OF LIVER SPECIFIC ANTIGEN ALLEVIATES LIVER TRANSPLANT REJECTION |
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Authors: | Jia Changku Zheng Shusen and Zhu Youfa |
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Institution: | Department of Hepatobiliary Pancreatic Surgery, Key Lab of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital of College of Medicine, Zhejiang University, Hangzhou 310003;Department of Pathology, College of Medicine, Zhejiang University, Hangzhou 310003 |
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Abstract: | OBJECTIVE: To study the effects of liver specific antigen (LSA) on liver allotransplantation rejection. METHODS: Orthotopic liver transplantation was performed in this study. Group I: syngeneic control (Wistar-to-Wistar); Group II: acute rejection (SD-to-Wistar). Group III: thymic inoculation of SD rat LSA day 7 before transplantation. The observation of general condition and survival time, rejection grades and the NF-kappaB activity of splenocytes were used to analyze severity of acute rejection and immune state of animals in different groups. RESULTS: The general condition of group I was fair post transplantation with no sign of rejection. All recipients of group II died within days 9 to 13 post transplantation with median survival time of 10.7 +/- 1.37 days. As for group III, 5 out of 6 recipients survived for a long period with remarkably better general condition than that of group II. Its rejection grades were significantly lower than group II (P<0.05). NF-kappaB activity was only detected in group I between days 5 and 7 after transplantation, whereas high activity of NF-kappaB was detected at all points in group II and low NF-kappaB activity was detected in group III which was significantly lower than that of group II (P<0.05). CONCLUSIONS: LSA is an important transplantation antigen directly involved in the immunorejection of liver transplantation. Intrathymic inoculation of LSA can alleviate the rejection of liver allotransplantation, grafts survive for a period of time thereby, allowing a novel way to liver transplantation immunotolerance. |
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Keywords: | liver specific antigen liver transplantation immunotolerance REJECTION LIVER TRANSPLANT LIVER SPECIFIC ANTIGEN novel immunotolerance survive long period time antigen high lower better median fair post sign Results activity splenocytes used |
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