APOBEC3G在慢性乙型肝炎、肝癌组织中的表达及其意义

目的 探讨APOBEC3G在慢性乙型肝炎和肝癌组织中的表达,及与慢性乙型肝炎和肝癌的关系.方法 应用免疫组织化学方法检测45例肝组织(正常肝组织8例,慢性乙型肝炎肝组织6例,肝癌组织21例,癌旁组织10例)中APOBEC3G的表达,采用半定量积分法判断表达的强度.结果 APOBEC3G在正常肝组织中无表达或仅见少量表达(表达阳性率12.5%),而在慢性乙型肝炎肝组织中、肝癌组织中和癌旁组织中均有较强表达(表达阳性率分别为83.3%、66.7%、50.O%),其差异有统计学意义(P<0.01).APOBEC3G在肝组织中的表达强度与同组中HBsAg、HBcAg的表达强度无明显相关(P>0.05).结论 慢性HBV感染能促使肝细胞内APOBEC3G表达增高,但APOBEC3G高水平的表达与HBsAg、HBcAg的表达强度无明显相关.

Expressions of APOBEC3G in liver tissues of chronic hepatitis B and hepatocellular carcinoma and its significance

Objective To examine the expression of APOBEC3G in liver tissues,including nor-mal control,chronic hepatitis B, hepatocellular carcinoma and para-eancerous tissue, and to investigate the relationship between the expression of APOBEC3G and hepatitis B virus infection. Methods Immunohis-tochemistry was used to detect the expression levels of APOBEC3G in liver tissues of 45 cases (8 healthy controls,6 eases of chronic hepatitis B,21 cases of hepatocellular carcinoma and 10 cases of para-cancer-ous tissues). The expression intensity of APOBEC3G was determined by using semiquantitative imegration methods. Results APOBEC3G expression levels in liver tissues of chronic hepatitis B, bepatocellular car-cinoma and para-cancer (positive rate was 83.3% ,66.7% and 50.0% respectively) were higher than that in healthy controls (positive rate was 12.5%) (P < 0.01). There was no relationship between the ex-pression levels of APOBEC3G and HBsAg or HBcAg (P > 0.05). Conclusion HBV infection could in-crease the expression levels of APOBEC3G,but the overexpression of APOBEC3G is not associated with the expression of HBsAg and HBcAg.