| 新生大鼠小脑内发育相关基因的筛选 |
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| 引用本文： | 李学礼 吕立夏 等. 新生大鼠小脑内发育相关基因的筛选[J]. 中国神经科学杂志, 2002, 18(2): 507-510 |
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| 作者姓名： | 李学礼 吕立夏 等 |
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| 作者单位： | 同济大学医学院生物化学教研室，同济大学医学院生物化学教研室，同济大学医学院生物化学教研室，同济大学医学院生物化学教研室，同济大学医学院生物化学教研室，同济大学医学院生物化学教研室，同济大学医学院生物化学教研室 上海200070，上海200070，上海200070，上海200070 |
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| 基金项目： | 铁道部科技发展专项基金 (J 99Z0 2 3 )资助 |
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| 摘 要： | 为筛选脑发育相关的基因 ,应用新发展的抑制消减杂交技术 ,以新生大鼠小脑mRNA逆转录cDNA作为测试者 (tester) ,成年大鼠小脑mRNA逆转录cDNA作为驱动者 (driver) ,进行消减杂交 ,克隆制备成新生大鼠小脑高表达的cDNA文库。挑选出 5 0个重组质粒 ,测序得到 37个不同基因片段序列。再次用反Northern杂交确认有意义的差异表达基因。同时将测序结果与GenBank注册序列进行同源性比较 ,2 0个大鼠中首次测得的基因表达序列标签 (EST)已被GenBank收录 (BG6 95 72 6 -BG6 95 74 5 )。
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| 关 键 词： | 表达序列标签 基因筛选 小脑 新生大鼠 |
Screening of developmental-associated genes in cerebellum of 1 day postnatal rat |
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| LI Xue li,LU Li xia,YANG Cui xiang,XU Lei,WU Xiao chun,XIANG Juan,WANG Yao. Screening of developmental-associated genes in cerebellum of 1 day postnatal rat[J]. Neuroscience Bulletin, 2002, 18(2): 507-510 |
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| Authors: | LI Xue li LU Li xia YANG Cui xiang XU Lei WU Xiao chun XIANG Juan WANG Yao |
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| Abstract: | For the purpose of screening developmental associated genes in cerebellum of rat, suppression subtractive hybridization (SSH) was carried out. The cDNAs from cerebellum of 1 day postnatal rat were taken as "tester" , and those from cerebellum of adult rat as "driver". After SSH and cloning, a differential cDNA library was constructed.Fifty of clones were selected out and sequenced. Thirty seven unique cDNAs were obtained except those with identical sequence. The expression of these cDNAs were estimated by reverse Northern assay.Twenty out of 37 cDNAs were identified to be new cDNAs that were found in rat at the first time and the gene sequence numbers were given by GenBank(BG695726 BG695745). [ |
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| Keywords: | expressed sequence tag gene screening cerebellum rat of postnatal 1 day |
| 本文献已被 CNKI 维普 等数据库收录！ |
