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| RP-HPLC法同时测定人血浆中氯氮平、喹硫平浓度 | |
| 引用本文： | 刘伟忠,陈清霞,朱军,温预关,黄璞.RP-HPLC法同时测定人血浆中氯氮平、喹硫平浓度[J].中国药师,2010,13(7):930-932. |
| 作者姓名： | 刘伟忠 陈清霞 朱军 温预关 黄璞 |
| 作者单位： | 1. 广州市脑科医院国家药品临床研究基地,广州,510370 2. 中山大学附属第二医院药学部 3. 华中科技大学同济医学院附属协和医院药学部 |
| 基金项目： | 广东省自然科学基金资助项目 |
| 摘 要： | 目的：建立同时测定人血浆中氯氮平、喹硫平浓度的反相高效液相色谱法.方法：以DiamonsilTM C18反相柱（150 mm×4.6 mm,5μm）为色谱柱,流动相为0.03 mol·L-1醋酸铵-甲醇（25∶75）;流速：0.8 ml·min-1;柱温：40 ℃;检测波长：254 nm.以乙酸乙酯与二氯甲烷（80∶20）为提取剂.结果：氯氮平在20.0～1280.0 ng·ml-1、喹硫平在25.0～1 600.0 ng·ml-1浓度范围内,峰面积与其浓度呈良好线性关系;氯氮平、喹硫平的高、中、低3种浓度相对平均回收率分别为97.47%,98.73%,100.82%和98.17%,98.75%,101.26%,提取回收率分别为79.82%,81.37%,83.25%和86.61%,82.55%,80.02%;日内、日间RSD均低于7%（n=5）.结论：该方法灵敏、准确、简单、快速,可用于临床血药浓度监测和药动学研究. |
| 关 键 词： | 氯氮平 喹硫平 血药浓度 反相高效液相色谱法 |
Determination of the Concentration of Clozapine and Quetiapine in Human Plasma by RP-HPLC with UV Detection |
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| Liu Weizhong,Chen Qingxia,Zhu Jun,Wen Yuguan,Huang Pu.Determination of the Concentration of Clozapine and Quetiapine in Human Plasma by RP-HPLC with UV Detection[J].China Pharmacist,2010,13(7):930-932. | |
| Authors: | Liu Weizhong Chen Qingxia Zhu Jun Wen Yuguan Huang Pu |
| Institution: | Liu Weizhong~1,Chen Qingxia~2,Zhu Jun~2,Wen Yuguan~1,Huang Pu~3(1.Base of Drug Clinical Study,Guangzhou Brain Hospital,Guangzhou 510370,China 2.Department of pharmacy,Second Affiliated Hospital,Sun Yat-sen University,3.Department of pharmacy,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology) |
| Abstract: | Objective： To establish a method for determination the concentration of clozapine and quetiapine in human plasma by RP- ItPLC. Method： The drug from plasma was analyzed in a reverse phase HPLC system C18 colunm （150 mm ×4.6 mm,5μm） ; mobile phase consisted of 0. 03 mol .L-1 ammonioum-methanol （25： 75） ; the flow rate was 0. 8 ml. min-1 ; the detection wavelength was at 254 nm. Ethyl acetate and dichloromethane （80： 20）was used as extracting solvent. Result： The calibration curves were linear in the range of 20. 0-1 280. 0 ng·ml-1 for clozapine ,25.0-1 600. 0 ng·ml-1 for quetiapine respectively. The average recoveries of clozapine and quetiapine in high ,middle and low concentrations were 97.47% ,98.73%, 100. 82% and 98. 17% ,98.75%, 101.26%, respectively; the extraction recovery were 79. 82% ,81.37% ,83.25% and 86. 61% ,82. 55% ,80. 02% ,respectively. The intra-day and inter-day variation（RSD） was less than 7% （n =5）. Conclusion： The method is sensitive,accurate,easy and quick,which can be used for clinical drug monitoring and pharmacokinetics studies. |
| Keywords: | Clozapine Quetiapine Plasma concentration RP-HPLC |
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