糖化终末产物对INS-1细胞基质金属蛋白酶2表达的影响

目的 探讨糖化终末产物(AGEs)对大鼠胰岛细胞株INS-1基质金属蛋白酶2(MMP-2)表达量的影响.方法 培养INS-1细胞,检测MMP-2表达;制备糖化胎牛血清,通过流式细胞仪检测细胞内活性氧簇(ROS)水平,通过逆转录PCR、实时定量PCR和Western印迹方法观察细胞中MMP-2表达量的变化.结果 INS-1细胞中存在MMP-2的表达;AGEs刺激后ROS明显升高(P<0.05),刺激组细胞内MMP-2mRNA表达量和蛋白表达水平均显著高于对照组(P<0.05).结论 提示胰岛β细胞内存在MMP-2表达,AGEs可诱导β细胞MMP-2表达量增高,MMP-2在介导胰岛氧化应激损伤中可能起着重要作用.

Effects of advanced glycation end-products on matrix metalloproteinase 2 expression in INS-1 cells

Objective To explore the impact of advanced glycation end-products ( AGEs) on matrix metalloproteinase 2 ( MMP-2) expression in cultivated INS-1 cells. Method INS-1 cells were cultivated and MMP-2 expression was analyzed. Glycated serum was prepared for incubating with INS-1 cell. Reactive oxygen species (ROS) was detected by flow cytometry. The intracelluar MMP-2 expression was analyzed by RT-PCR, realtime PCR and Western blot. The MMP-2 cDNA was expressed in cultivated INS-1 cells. Result The level of ROS treated with AGEs was significantly higher than that in the control( P<0.05 ) , and the levels of MMP-2 and its protein expressions turned out as well( P<0. 05). Conclusion The results suggest that MMP-2 was expressed in INS-1 cells. Increased MMP-2 expression in ?cells may be induced by AGEs, suggesting that MMP-2 might play an important role in oxidative stress-mediated islet injury.