| 核内M-CSF对NIH3T3细胞内微丝的影响 |
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| 引用本文： | 张晓红,唐圣松,赵飞骏,刘月顺,龙治锋. 核内M-CSF对NIH3T3细胞内微丝的影响[J]. 解剖科学进展, 2007, 13(4): 314-316,320 |
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| 作者姓名： | 张晓红 唐圣松 赵飞骏 刘月顺 龙治锋 |
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| 作者单位： | 南华大学,组织胚胎学教研室,湖南,衡阳,421001;南华大学,药物药理研究所,湖南,衡阳,421001;南华大学,病原生物学研究所,湖南,衡阳,421001 |
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| 基金项目： | 国家自然科学基金(30270684)，湖南省杰出青年基金(02JJYB004)，湖南省自然科学基金重点项目(04JJ20006) |
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| 摘 要： | 目的建立M-CSF核内稳定表达的NIH3T3细胞系,探讨核内M-CSF对细胞骨架的影响。方法经脂质体介导核内定位表达重组体pCMV/M-CSF转染NIH3T3细胞,G418筛选后,用Rt-PCR、免疫细胞化学鉴定其在真核细胞中的表达及定位分布,用考马斯亮蓝染色细胞微丝测定M-CSF进入细胞核后对细胞骨架的影响。结果RT-PCR结果显示转染pCMV/M-CSF的NIH3T3细胞能稳定表达M-CSFmRNA;免疫细胞化学结果显示表达的M-CSF定位于NIH3T3细胞核。考马斯亮蓝染色结果显示转染pCMV/M-CSF的NIH3T3细胞内微丝数量减少,排列紊乱。结论核内M-CSF可引起NIH3T3细胞内微丝数量减少,排列紊乱。
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| 关 键 词： | 巨噬细胞集落刺激因子(M-CSF) 核内定位表达 NIH3T3细胞 微丝 |
| 文章编号： | 1006-2947（2007）04-0314-03 |
| 收稿时间： | 2007-05-20 |
| 修稿时间： | 2007-05-20 |
The Effect of M-CSF in Nucleus on Microfilaments in NIH3T3 Cells |
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| ZHANG Xiao-hong,TANG Sheng-song,ZHAO Fei-jun,LIU Yue-shun,LONG Zhi-feng. The Effect of M-CSF in Nucleus on Microfilaments in NIH3T3 Cells[J]. Progress of Anatomical Sciences, 2007, 13(4): 314-316,320 |
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| Authors: | ZHANG Xiao-hong TANG Sheng-song ZHAO Fei-jun LIU Yue-shun LONG Zhi-feng |
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| Abstract: | Objective To establish a cell line which stably expresses macrophage colony-stimulated factor (M-CSF) in NIH3T3 cells nucleus and explore the effect of nuclear M-CSF on the cytoskeleton in NIH3T3 cells. Methods Using liposome, the pCMV/M-CSF was transfected into NIH3T3 cells and the cells clones were selected with G418. The expression and localization of M-CSF in NIH3T3 cells were verified by RT-PCR and immunocytochemistry. The effect of nuclear M-CSF on cytoskeleton was analyzed by dying with Coomassie brilliant blue. Result After transfecting pCMV/M-CSF into NIN3T3 cells by liposome and screening using G418, the pCMV/M-CSF-transfected NIN3T3 cells can stably express both M-CSF mRNA and protein in nucleus. Less and disorderly-arranged microfilaments were found in pCMV/M-CSF-transfected NIN3T3 cells, compared to un-transfected NIN3T3 cells. Conclusion Nuclear M-CSF gives rise to the less and disorderly-arranged microfilaments in NIH3T3 cells. |
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| Keywords: | macrophage colony-stimulating factor nucleus-localization expression microfilament NIH3T3 cell line |
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