体外血脑屏障模型的建立

目的:利用自发转化的人脐静脉内皮细胞系ECV304和分离纯化的大鼠星形胶质细胞共培养试图建立一种具有重复性好、细胞纯度高、培养操作简便、接近在体状态的体外血脑屏障模型。方法:分3步:(1)分离纯化培养大鼠星形胶质细胞;(2)建立体外血脑屏障BBB模型(内皮细胞系/星形胶质细胞非接触共培养);(3)模型鉴定。结果:利用ECV304与原代培养的星形胶质细胞共培养建立的血脑屏障模型无论是在形态学、屏障功能上还是内皮细胞的特异性标志物的表达水平上都与在体血脑屏障的特性相似。结论:可以利用ECV304与原代培养的星形胶质细胞共培养建立体外血脑屏障模型。

The Model of Blood Brain Barrier(BBB) Established in vitro

Objective: To establish blood brain barrier(BBB) model in vitro by way of cocultivation of human endothelial cells of vein(ECV304) and astrocytes of rat.This model should be easier repeated and more similar to the BBB in vivo,and the cell should have more degree of purity.Methods: There were three procedures:(1) to dissociate,pure and culture rat's astrocytes;(2)to establish blood brain barrier(BBB) model in vitro(endothelial cells/ astrocytes untouch cultured);(3)to assay model.Results:The BBB model in vitro established by way of cocultivation of ECV 304 and astrocytes was similar to internal blood brain barrier(BBB)in vitro,either in morphology,barrier function or in expression of endothelial cells specificity label.Conclusion:The BBB model in vitro can be established by way of cocultivation of ECV 304 and astrocytes.