慢性牙周炎龈下菌斑中五种牙周可疑致病微生物的分布

目的研究五种牙周可疑致病微生物在慢性牙周炎患者龈下菌斑的分布。方法选择27例慢性牙周炎患者，每位患者选取牙周袋最深的两个位点作为观察位点，采集龈下微生物样本，采用多重聚合酶链反应和反杂交的方法对伴放线菌嗜血菌、牙龈卟啉单胞菌、福赛斯坦纳菌、中间普雷沃菌和齿垢密螺旋体五种微生物进行半定量检测。结果在所检测的54个位点中，牙龈卟啉单胞菌、中间普雷沃菌、福赛斯坦纳菌和齿垢密螺旋体均有较高的检出率，分别为98.15%、92.59%、100%和98.15%；伴放线菌嗜血菌检出率较低，为20.37%。牙龈卟啉单胞菌和福赛斯坦纳菌的检出量明显高于其他三种微生物，其差异有统计学意义（P<0.05）。结论慢性牙周炎患者多存在牙龈卟啉单胞菌、福赛斯坦纳菌、中间普雷沃菌和齿垢密螺旋体的同时感染，且牙龈卟啉单胞菌和福赛斯坦纳菌的感染量较高。

Distribution of five periodontal pathogens in subgingival plaque in chronic periodontitis

Objective To investigate the distribution of H. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythensis and T. denticola in the subgingival plaque in the patients with chronic periodontitis. Methods 27 pa-tients with chronic periodontitis were included. Two of the deepest pockets of each patient were selected as the study sites. Semi-quantification of subgingival microorganism samples was analyzed by multiplex polymerase chain reaction and reverse hybridization assay. Results P. gingivalis, P. intermedia, T. forsythensis and T. denticola were detected in a high proportion of examined sites(corresponding values were 98.15%, 92.59%, 100% and 98.15%), however, the pro-portion of H. actinomycetemcomitans was low(20.37%). The levels of P. gingivalis and T. forsythensis were higher than the other three microorganisms with statistical significance. Conclusion The simultaneous infection of P. gingivalis, T. forsythensis, P. intermedia and T. denticola is found in the patients with chronic periodontitis, in which the levels of P. gingivalis and T. forsythensis are higher than the other two microorganisms.