磁性附着体模拟静磁场对人牙周膜成纤维细胞的生物学效应研究

目的探讨磁性附着体静磁场对人牙周膜成纤维细胞（HPDLF）的生物学效应。方法对体外培养的HPDLF分别进行12.5、125和250 mT静磁场持续加载4 d，设无外加静磁场作用的对照组，分别测定各组细胞的碱性磷酸酶（ALP）和超氧化物歧化酶（SOD）活性，采用流式细胞仪行细胞周期分析。结果高剂量静磁场（250 mT）明显增强HPDLF的ALP活性（P<0.05）。各磁场剂量组的细胞SOD活性与对照组比较，差异无统计学意义（P>0.05）。细胞G0 /G1期、S期、G2 /M期分布和增殖指数与对照组相比也无统计学差异（P>0.05）。结论在本实验条件下磁性附着体静磁场可促进HPDLF的ALP活性，而对SOD活性以及细胞周期分布无明显影响。

Study of biologic effects of simulating static magnetic field of magnetic attachment on human periodontal ligament fibroblasts

Objective To evaluate the biologic effects of simulating static magnetic field of magnetic attachment on human periodontal ligament fibroblasts(HPDLF).Methods HPDLF were cultured in vitro and exposed to simu-lating static magnetic fields of magnetic attachments for 4 days with magnetic flux density of 12.5,125 and 250 mT respectively,while the control group free of additional static magnetic field was also set up.The activities of cellular alkaline phosphatase(ALP) and superoxide dismutase(SOD) were measured with a spectrophotometer,and cell cycle was analyzed by flow cytometry.Results An increase were detected,induced by 250 mT static magnetic field exposure for 4 days in terms of activity of ALP in HPDLF,as compared with the control group(P<0.05).However,no corresponding effects were found in activity measurements of SOD(P>0.05).Also there were no statistical differences observed on cell cycle distribution(P>0.05) or cell proliferation index(P>0.05) under such static magnetic fields.Conclusion The static magnetic fields of magnetic attachment used in this study have improved the activity of ALP,and do little inference to the activity of SOD and the cell cycle distribution.