渐进性咬合紊乱对大鼠髁突软骨碱性成纤维细胞生长因子表达的影响

目的探讨渐进性咬合紊乱大鼠髁突软骨中碱性成纤维细胞生长因子（bFGF）表达的变化规律。方法建立大鼠渐进性咬合紊乱动物模型，应用免疫组织化学SABC方法检测髁突软骨中bFGF的表达，并通过计算阳性细胞密度来探讨bFGF在不同实验时间点的表达变化情况。结果bFGF主要表达在大鼠髁突软骨的增殖层、过渡层和肥大层。对照组大鼠髁突软骨中从6周龄到10周龄bFGF的表达逐渐增强，10周龄后降低并保持在一个稳定的水平；成年实验组和幼年实验组在实验2、6、8周时，bFGF的表达均高于各自的对照组，具有统计学意义（P<0.05），而实验4周时与对照组之间无统计学差异。结论bFGF参与了大鼠渐进性咬合紊乱所致髁突软骨的改建活动。

Effect of Gradually Induced Occlusal Disorders on the Expression of Basic Fibroblast Growth Factor of Condylar Cartilage in Rat

Objective To study the effect of gradually induced occlusal disorders on the expression of basic fi-broblast growth factor(bFGF) of condylar cartilage in rat.Methods The model of gradually induced occlusal disor-ders was established in rat.The expression of bFGF was examined by SABC technique of immunohistochemistry.The expression of bFGF was analyzed by amount of positive cells.Results bFGF was expressed positively in the prolif-erative cell layer,maturative layer and hypertrophical cell layer in the rat mandibular condyle cartilage.In control group,expression of bFGF increased from 2-week-old to 6-week-old,then it had a decrease during experiment.Compared with the control group,bFGF of experiment group was increasing at 2 week,6 week and 8 week during experiment.But there was decreaseing at 4 week.There was no difference between young experiment group and the adult experiment group.Conclusion The gradually induced occlusal disorders may lead to significant increase of expressiong of bFGF in condyle cartilage,which suggests that the bFGF may be involve in the procedure of repairing process of articular cartilage.