腺病毒介导的骨形态发生蛋白2基因转染肌卫星细胞诱导成骨的动物实验研究

目的观察腺病毒介导的BMP- 2基因转染肌卫星细胞后体内骨诱导能力。方法采用动物实验方法，将转染BMP- 2基因的绿色荧光蛋白（GFP）转基因鼠肌卫星细胞吸收到胶原支架上，然后将细胞胶原支架复合物移植入野生型129sv小鼠的后小腿肌肉中，应用X线片观察、组织学检查及荧光显微镜观察转染细胞的诱导成骨能力。结果用BMP- 2基因转染的肌卫星细胞复合胶原支架移植入野生型小鼠后小腿筋膜下肌肉后会产生异位骨形成，移植2周后其中有GFP阳性的成骨细胞和骨细胞，3周后形成良好的骨组织，4周时已形成成熟的骨组织，而且骨组织具有GFP荧光。对照组用胶原支架和未转染的肌卫星细胞移植，未出现诱导性异位骨形成。结论Ad- BMP2转染的肌卫星细胞以胶原为支架可以在肌肉内诱导骨组织形成。

Ectopic bone induction in vivo after transplantation of skeletal satellite cells from green fluorescence protein transgenic mouse transfected by adenoviral vectors encoding bone morphogenetic protein-2

Objective To observe the ability of induced ectopic bone using skeletal muscles satellite cells(SMSCs) from newborn green fluorescence protein(GFP) transgenic mice mediated by Ad-BMP2. Methods Transplantation of SMSCs transduced with Ad-BMP2 into back lamb muscles of subfascia in wildtype 129sv mice with a complex of collagen scaffords, then the tissue histologic examination, X ray plain film, fluorescence microscopy were used. Results Transplantation of SMSCs transfected with Ad-BMP2 into back lamb muscles of subfascia generated ectopic bone formation involving GFP-positive osteoblasts and osteocytes 2 weeks and mature bone formation 4 weeks after transplantation. SMSCs non-transfected with Ad-BMP2 failed to induce ectopic bone formation. Conclusion SMSCs retain differentiation potentitality into osteoblasts in response to Ad-BMP2. They are useful tools for analyzing the process of osteoblast differentiation in vivo after transplantation.