慢性牙周炎患者龈下菌斑中福赛斯坦纳菌与临床指标的关系

目的分析慢性牙周炎患者龈下菌斑中福赛斯坦纳菌（T.forsythensis）的分布情况，及其与牙周临床指标的关系，探讨T.forsythensis与慢性牙周炎发生发展的关系。方法采集108例慢性牙周炎患者的216个病变位点和108个健康位点的龈下菌斑，共324个样本，采用16S rRNA聚合酶链反应检测T.forsythensis的分布情况。对324个取样位点进行临床检查，记录探诊深度、临床附着丧失和探诊出血情况，分析T.forsythensis与临床指标的相互关系。结果慢性牙周炎患者病变位点与健康位点T.forsythensis的检出率分别为59.72%和3.70%，二者间有统计学差异（P<0.001）。随着探诊深度和临床附着丧失的增加，T.forsythensis的检出率也升高，Spearman相关系数分别为0.48和0.51。探诊出血阳性位点T.forsythensis的检出率为61.31%，明显高于探诊出血阴性位点的检出率8.80%（P<0.001）。结论T.forsythensis与慢性牙周炎发生、发展关系密切。

Prevalence of Tannerella forsythensis in Subgingival Microflora of Chinese Chronic Periodontitis Patients

Objective The aim of this study was to investigate the prevalence of T.forsythensis in Chinese chronic periodontitis patients,and correlate it with clinical parameters.Methods 108 chronic periodontitis patients were selected according to clinical criteria.In each patient,subgingival plaque samples were collected from two of the periodontitis involved molar sites with the deepest periodontal pocket depth(diseased sites) and one healthy gingival sulcus(healthy sites).T.forsythensis was detected by 16S rRNA polymerase chain reaction.Clinical assessments in-cluding probing depth,clinical attachment loss and bleeding on probing,were made at sampling sites.Results The prevalence of T.forsythensis in diseased sites and healthy sites was 59.72% and 3.70% respectively(P<0.001). The prevalence of T.forsythensis was significantly higher in deeper pockets and more serious clinical attachment loss,Spearman rank correlation coefficient was 0.48 and 0.51 respectively.The prevalence of T.forsythensis in BOP( )sites was 61.31%,and in BOP(-) sites was 8.80%(P<0.001).Conclusion The prevalence of T.forsythensis was significantly correlated with clinical parameters,suggesting that T.forsythensis is closely related to chronic peri-odontitis in the Chinese population.