复方中药治疗非酒精性脂肪性肝病的实验研究

目的观察复方中药治疗非酒精性脂肪性肝病的疗效和作用机制。方法运用高脂饲料饮食制备非酒精性脂肪性肝病动物模型,治疗组药物灌胃,采用酶法测定血及肝组织中总胆固醇(TC)、甘油三酯(TG)、游离脂肪酸(FFA)及血中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)的含量,观察肝组织学变化。采用反转录-聚合酶链反应(RT-PCR)测定酰基辅酶A氧化酶(AOX)的表达。结果中药高剂量组血及肝中TC、TG、FFA含量较模型组有明显降低(P<0.05);中药高剂量组ALT、AST较模型组有明显降低(P<0.05);中药治疗组肝组织脂肪变及炎症计分较模型组有明显降低(P<0.05);AOX的表达中药组和吉非贝齐组较模型组明显升高(P<0.05)。结论中药治疗非酒精性脂肪性肝病有明显疗效,可能通过激活过氧化物酶体增生物激活受体-α(PPAR-α)使AOX表达上调而改善脂肪变。

Experimental study on treatment of nonalcoholic fatty liver disease by compound herbs

Objective To investigate the effect of compound herbs in nonalcoholic fatty liver disease. Methods Fatty liver animal model was duplicated with high fat diet,and divided into model group,herbs high dose group,herbs low dose group,gemfibrozil group and normal control group randomly.Each treatment group was intervened by its own medicine.Then the contents of total cholesterol(TC),triglycerides (TG),free fatty acid(FFA) in serum,the contents of TC,TG,FFA and the expression of acyl-CoA oxidase(AOX) in hepatic tissue were detected,evaluating the levels of steatosis and steatohepatitis in liver. Results Compared with the model group(7.33±0.42,1.49±0.06,888.23±56.15,244.75± 30.27,108.78±6.95,166.54±6.54),the content of serum lipid and liver lipid in herbs high dose group(2.46±0.10,0.63±0.06,279.01±13.43,89.82±2.78,53.22±2.39,97.30±9.91) were reduced remarkably(q=45.18,38.39,51.73,29.63,29.46,P<0.05).The severity degrees of steatosis and steatohepatitis were significantly lower in herbs high dose group and gemfibrozil group than those in model group(P<0.05).The expression of AOX was higher in herbs high dose group and gemfibrozil group than in model group(q=8.46,4.04,P<0.05). Conclusion The compound herbs is effective in treating nonalcoholic fatty liver disease.