| 羟氯喹对梗死区周边心肌共济失调毛细管扩张突变基因下游分子及凋亡的影响 |
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| 引用本文： | 周婧,李刚.羟氯喹对梗死区周边心肌共济失调毛细管扩张突变基因下游分子及凋亡的影响[J].临床心血管病杂志,2010,26(5). |
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| 作者姓名： | 周婧 李刚 |
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| 作者单位： | 重庆医科大学附属第一医院老年科心血管病组,重庆,400016 |
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| 基金项目： | 重庆市科委自然科学基金[渝科发计字(2007)25号CSTC,2007BB5276];;重庆市卫生局医学科学技术研究项目[渝卫教2004(53)04-2-154,2009-2-290] |
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| 摘 要： | 目的:探讨羟氯喹对大鼠梗死区周边心肌共济失调毛细管扩张突变基因(ATM)下游信号分子及凋亡的影响。方法:按Maisel方法建立心肌梗死与假手术模型,将术后3 d仍存活的SD大鼠按不同干预条件分为心肌梗死羟氯喹组(A组,n=11),心肌梗死安慰剂组(B组,n=15),假手术羟氯喹组(C组,n=7),假手术安慰剂组(D组,n=7)。计算处理3个月后各组死亡率,原位TUNEL法检测心肌细胞凋亡,Western Blot测定梗死区周边心肌MMP-9、P-Akt、P53蛋白表达。结果:A、B、C、D组大鼠处理3个月后死亡率分别为9.0%、40.0%、0%、0%,心肌细胞凋亡率分别为(12±4)%、(30±5)%、(2±2)%、(3±2)%。A组与B组心肌MMP-9蛋白IA分别为0.67±0.11、1.07±0.13,P<0.05;P-Akt蛋白IA分别为1.12±0.21、0.58±0.18,P<0.05;P53蛋白IA分别为1.01±0.20、0.98±0.19,P>0.05。C组与D组心肌MMP-9蛋白IA分别为0.42±0.14、0.56±0.16,P>0.05;P-Akt蛋白IA分别为0.78±0.20、0.6...
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| 关 键 词： | 心肌梗死 凋亡 羟氯喹 共济失调毛细管扩张突变基因 |
Effect of hydroxychloroquine on downstream proteins of ATM in myocardium and apoptosis of cardiomyocytes around the infarction zone in rats |
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| ZHOU Jing,LI Gang.Effect of hydroxychloroquine on downstream proteins of ATM in myocardium and apoptosis of cardiomyocytes around the infarction zone in rats[J].Journal of Clinical Cardiology,2010,26(5). |
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| Authors: | ZHOU Jing LI Gang |
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| Institution: | Division of Cardiology/a>;Department of Gerontology/a>;the First Affiliated Hospital/a>;Chongqing Medical Univercity/a>;Chongqing/a>;400016/a>;China |
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| Abstract: | Objective:To investigate the effect of hydroxychloroquine on downstream protein of ataxia telangiectasia mutated(ATM) in myocardium and apoptosis of cardiomyocytes around the infarction zone in rats.Method:Myocardial infarction(MI) and sham-operation in rats were established by Maisel's method.Three days after operation,surviving Sprague-Dawley(SD) rats were divided into MI hydroxychloroquine group(n=11,group A),MI placebo group(n=15,group B),sham-operation hydroxychloroquine group(n=7,group C),sham-operati... |
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| Keywords: | myocardial infarction apoptosis hydroxychloroquine ataxia telangiectasia mutated |
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