黄芩茎叶总黄酮对大鼠实验性高脂血症的预防作用

背景黄芩是一味常用中药,传统药用其根,茎叶一0是被废弃,为充分利用黄芩茎叶资源,本研究所对黄芩茎叶的药理药化进行了系统研究.目的探讨黄芩茎叶的主要药效部位总黄酮对大鼠实验性高脂血症的预防作用.设计随机对照实验.单位承德医学院中药研究所.对象实验于1999-03/2000-01在承德医学院中药研究所完成.wistar大鼠60只,雄性,初始体质量(200±10)g,由中国医学科学院实验动物繁育场提供,合格证号01-3008.干预大鼠60只被随机分为6组,每组10只,正常对照组,高脂模型组,总黄酮小、中、大剂量组(剂量分别为12.5,25,50 mg/kg)和氯苯丁酯组(剂量为25 mg/kg).正常对照组饲喂基础饲料,高脂模型组饲喂高脂饲料,总黄酮大、中、小剂量组和氯苯丁酯组,在喂高脂饲料的同时给予相应剂量的药物.连续给药30 d,观察大鼠的血脂变化.主要观察指标应用CL-7200型全自动生化分析仪,检测大鼠血清总胆固醇、三酰甘油、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇的含量,并计算动脉粥样硬化指数(总胆固醇-高密度脂蛋白/高密度脂蛋白).结果纳入60只大鼠均进入结果分析.①大鼠血清总胆固醇含量高脂模型组明显高于正常对照组[(5.01±1.05,2.33±0.35)mmol/L,(P＜0.01)];总黄酮小、中、大剂量组分别为(4.15±1.12,3.03±0.31,2.98±0.56)mmol/L,小剂量组与模型组比较差异无显著性(t=1.74,P＞0.05),中、大剂量组与模型组比较差异显著(t=5.66～5.23,P＜0.01).②大鼠血清三酰甘油的含量总黄酮小、中、大剂量组分别为[(1.22±0.56,1.56±0.41,1.24±0.45)mmol/L],与模型组[(2.14±0.74)mmol/L]比较差异显著(t=2.19～3.45,P＜0.05～0.01).③大鼠血清低密度脂蛋白胆固醇含量总黄酮小、中、大剂量组分别为[(2.67±0.45,1.41±0.23,1.29±0.23)mmol/L],与模型组[(3.94±0.42)mmol/L]比较差异显著(t=5.77～12.71,P＜0.05～0.01].④大鼠血清高密度脂蛋白胆固醇含量总黄酮小、中、大剂量组分别为[(0.72±0.23,0.91±0 32,1.05±0.23)mmol/L],小剂量组与模型组[(0.56±0.21)mmol/L]比较差异无显著性(t=1.51,P＞0.05),中、大剂量组与模型组比较差异显著(t=2.92～4.38,P＜0.05～0.01).⑤动脉粥样硬化指数总黄酮小、中、大剂量组分别为(2.96±1.35,2.10±0.97,1.55±0.41),与模型组(4.23±0.65)比较差异显著(t=3.54～9.49,P＜0.01).结论黄芩茎叶总黄酮对大鼠长期喂以高脂饲料所造成的血清总胆固醇、三酰甘油、低密度脂蛋白胆固醇的升高有明显的抑制作用,并使高密度脂蛋白胆固醇的含量有一定程度的升高,表明总黄酮对大鼠实验性高脂血症有明显的预防作用.

Preventive effect of total flavones from stem and leaf of scutellaria baicalensis on experimental hyperlipidemia in rats

BACKGROUND: The Scutellaria baicalensis is a traditional Chinese herb frequently used. Only its root is used, and its stem and leaf are abandoned in traditional custom. Inorder to make full use of medicinal material resource, the chemical component and pharmacological effects of the stem and leaf have been studied.OBJECTIVE: To observe the preventive effect of total flavones from stem and leaf of scutellaria baicalensis on experimental hyperlipidemia in rats.DESIGN: Randomized controlled trial.SETTING: Institute of Traditional Chinese Medicine, Chengde Medical CollegeMATERIALS: The experiment was conducted in the Institute of Traditional Chinese Medicine, Chengde Medical College from March 1999 to January 2000. Totally 60 male Wistar rats with beginning body mass(200±10)g were provided by Experimental Animal Research Institute, Chinese Academy of Medical Science (Qualification No.01-3008).INTERVENTIONS: Sixty rats were randomly divided into 6 groups:normal control group, high-lipid model group, the groups of 12.5, 25 and 50 mg/kg of total flavonoids (TF group) and clofibrate group (25 mg/kg),with 10 rats in each group. The rats in the normal control group were fed with basic feed. The rats in the high-lipid model group were fed with highlipid feed. Rats in the TF group and clofibrate group were fed synchronously with high-lipid feed and total flavonoids or clofibrate for consecutive 30 days. The change of blood lipid was observed.MAIN OUTCOME MEASURES: The levels of total cholesterol (TC),triglyceride (TG), low density lipoprotein (LDL-C), high density lipoprotein (HDL-C) in the serum of all the rats were measured with CL-7200 type automatic biochemistry analytical instrument at the end of the experiment, and the atherosclerosis indexes (AI) were calculated (AI=TC-HDL-C/HDL-C).RESULTS: Totally 60 rats entered the result analysis. ① Level of TC in the serum of rats: The level in the high-lipid model group was significantly higher than that in the normal control group [(5.01 ±1.05,2.33±0.35)mmol/L, (P ＜ 0.01 )]; The level in the groups of 12.5 mg/kg, 25 mg/kg and 50 mg/kg of TF was (4.15±1.12, 3.03±0.31,2.98±0.56)mmol/L, there was no significant difference between group of 12.5 mg/kg of TF and model group (t=1.74, P ＞ 0.05), but there was significant difference between groups of 25 mg/kg and 50 mg/kg of TF and model group (t=5.66-5.23, P ＜ 0.01). ②Level of TG in the serum of rats: The level in the groups of 12.5 mg/kg, 25 mg/kg and 50 mg/kg of TF was (1.22±0.56)mmol/L,(1.56±0.41)mmol/L,(1.24 ±0.45)mmol/L respectively, compared with model group(2.14±0.74) mmol/L, there was significant difference (t =2.19-3.45, P ＜ 0.05-0.01). ③ LDL-C level of the serum in the rats: The level in the groups of 12.5 mg/kg, 25 mg/kg and 50 mg/kg of TF was (2.67 ±0.45) mmol/L, (1.41 ±0.23)mmol/L, (1.29±0.23) mmol/Lrespcrtively,compared with model group[(3.94±0.42)mmol/L, there was significant difference (t=5.77-12.71, P ＜ 0.05-0.01). ④ HDL-C level of the serum in the rats: the level in the groups of 12.5 mg/kg, 25 mg/kg and 50 mg/kg of TF was (0.72±0.23)mmol/L,( 0.91±0.32)mmoL/L,(1.05±0.23)mmoL/L respectively, there was no significant difference between group of 12.5 mg/kg of TF and model group[(0.56±0.21)mmol/L, but there was significant difference between groups of 25 mg/kg and 50 mg/kg of TF and model group (t=2.92-4.38,0.05 ,P ＜ 0.05-0.01).⑤AI: the level in the groups of 12.5 mg/kg, 25 mg/kg and 50 mg/kg of TF was(2.96 ±1.35), (2.10±0.97), (1.55±0.41)respectively, compared with model group (4.23±0.65) , there was significant difference (t =3.54-9.49 ,P ＜ 0.01).CONCLUSION: TF has significantly inhibitory effect on the increase of TC, TG and LDL-C in the serum of the rats induced by high-lipid feed; it can also increase the level of HDL-C, indicating TF has obviously preventive effect on experimental hyperlipidemia in rats.