Differentiation of porcine Brachyspira species by a novel nox PCR-based restriction fragment length polymorphism analysis |
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| Authors: | Rohde Judith Rothkamp Anja Gerlach Gerald F |
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| Institution: | Institut für Mikrobiologie und Tierseuchen, Tieraerztliche Hochschule Hannover, Hannover, Germany. Jurohde@gmx.de |
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| Abstract: | A novel PCR-based restriction fragment length polymorphism analysis of the Brachyspira nox gene was developed. The restriction patterns for Brachyspira hyodysenteriae, B. pilosicoli, B. intermedia, B. murdochii, and B. innocens were highly distinct with two restriction endonucleases only. The assay proved to be user-friendly and robust. |
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