绞股蓝提取物抗抑郁活性研究

目的考察绞股蓝提取物抗抑郁活性。方法通过3种动物模型,即小鼠悬尾实验(TST)、强迫游泳实验(FST)和开场实验(OFT)模型,首次测试绞股蓝75%乙醇提取物(醇提物,JE)及其水提物(JW)的抗抑郁活性。结果 JE(2.5,5 g/kg)可使小鼠在TST及FST的不动时间均显著性缩短(P<0.05),呈现出良好的量效关系,且与阳性药盐酸氟西汀(FH)相比无显著性差异(P>0.05),而JW(2.5,5 g/kg)对小鼠在TST及FST的不动时间均无显著性差异(P>0.05);各组小鼠OFT穿格次数比较,均无显著性差异(P>0.05)。结论首次证实绞股蓝醇提物具有抗抑郁活性,且与FH的作用相当,而其水提物在既定给药方案下无抗抑郁活性。

Antidepressant Activities of Extracts from Gynostemma pentaphyllum ( Thunb.) Makino

ObjectiveTo investigate the antidepressant effect of extracts from Gynostemma pentaphyllum(Thunb.) Makino(Cucurbitaceae)(GP) for the first time. MethodsThree models,the mouse forced swim test(FST),tail suspension test(TST) and open field test(OFT),were used to observe antidepressant-like activity of the 75% ethanol extract(JE) and the water extract(JW) isolated from GP. ResultsCompared with the model group(CMC-Na),JE(2.5,5 g/kg,ig) significantly reduced the immobility time in dose-dependent manners during the FST and the TST,while JW(2.5,5 g/kg,ig) did not.Meanwhile,there was no significant difference between JE and fluoxetine hydrochloride(FH). ConclusionJE isolated from GP exerts antidepressant activity and has the similar effect with the positive drug FH,while JW dose not.