延胡索和左旋延胡索乙素对大鼠吗啡条件性位置偏爱效应消退作用的比较

目的观察并比较延胡索及其有效成分左旋延胡索乙素(l-tetrahydropalmatine,l-THP)对大鼠吗啡条件性位置偏爱(conditioned place preference,CPP)效应消退时间的影响。方法剂量递增法建立大鼠吗啡CPP模型,雄性SD大鼠颈背部注射吗啡和生理盐水训练10 d,在12 d确认CPP模型建立成功后,连续21 d给予延胡索2,1,0.5 g.kg-1水提液(分别含l-THP0.153,0.077,0.038 mg),以及l-THP 3.76,1.88,0.94 mg.kg-1灌胃治疗,观察各组大鼠吗啡CPP效应的消退时间。结果与生理盐水对照组比较,吗啡模型组大鼠在伴药箱停留的时间明显延长(P<0.05);治疗后,延胡索2,1g.kg-1水提液和l-THP 3.76,1.88 mg.kg-1组的CPP效应在用药6 d消退,而模型组、延胡索0.5 g.kg-1和l-THP0.94 mg.kg-1组在用药第2天消退。结论延胡索和l-THP均能加速大鼠吗啡CPP效应的消退,但含一倍量l-THP单体的延胡索中药相当于单独应用约24倍l-THP单体的效果。

Comparison of the Effects between Corydalis Yanhusuo and L -tetrahydropalmatine on Morphine - induced Place Preference Extinction in Rats

ObjectiveTo observe and compare effects on conditioned place preference(CPP) extinction in morphine-induced rats between Corylalis Yanhusuo and L-telra-hyoropalmatine. MethodsThe model of CPP was established by escalating doses of morphine in rats.Male SD rats were hypodermically injected with morphine and saline for 10 days.Different doses(2,1 and 0.5 g·kg-1) of Corydalis yanhusuo(pure l-THP:0.153,0.077 and 0.038mg·kg-1) were applied to treat the rats for 21 days when the model of CPP was validated on the 12 th day.Another group was treated with l-THP(3.76,1.88 and 0.94 mg·kg-1).The extinction time of each group was recorded. ResultsCompared with NS group,the prolonged detention time of model group was notably observed in drug-paired compartment(P<0.05).Moreover,the reduction effects on CPP were observed in the groups of water extract(2,1 g·kg-1) and l-THP(3.76,1.88 mg·kg-1) subjected to medicine for 6 days(P<0.05).However,the similar effects were found among the model group,Corydalis yanhusuo group(0.5 g·kg-1) and l-THP(0.94 mg·kg-1) group after 21-day treatments(P<0.05). ConclusionCorydalis yanhusuo and l-THP can substantially accelerate the reduction effects in rats suffered from morphine.However,Corydalis yanhusuo is 24 times higher in efficacy than that of l-THP.