广西甜茶制剂中甜茶素含量的RSLC-DAD测定

目的建立RSLC-DAD测定广西甜茶制剂中甜茶素含量的方法。方法色谱柱为Dionex RSLC 120 C18柱(50 mm×2.1 mm,2.2μm),流动相为乙腈-水(36∶64),检测波长205 nm,柱温40℃,流速0.3 ml/min,进样量2μl。结果甜茶素的线性范围为0.03～0.6μg;加样回收率为99.1%,RSD为0.97%(n=9);甜茶素在21份中国和日本市售甜茶制剂中的含量范围为0～63.79 mg/g。结论该检测方法简便、快速、可靠,可作为测定甜茶制剂中甜茶素含量的方法。

Quantification of Rubusoside in Preparations of Rubus Suavissimus by RSLC - DAD

Objective To establish a rapid separation liquid chromatography with Diode Array Detecter(RSLC-DAD) method for quantification of rubusoside in Rubus Suavissimus preparations. Methods The separation was performed on a Dionex RSLC Acclaim 120 C18(50 mm×2.1mm,2.2μm) column with the mobile phase consisted of acetonitrile-water(36∶64) at the flow rate of 0.3 ml/min.The detection wavelength was set at 205nm,and the column temperature was kept constantly at 40 ℃.The injection volume was 2 μl. Results The linear range was 0.03~0.6μg for rubusoside.The average recovery was 99.1% with RSD of 0.97%(n=9).The content range of rubusoside were 0 ~ 63.79 mg/g in 21 Sweet tea preparations from China or Japan. Conclusion The method is simple,rapid,accurate,and helpful for quantification of rubusoside in R.Suavissimus preparations.