硒化壳聚糖促创面愈合作用的体内研究

目的:研究硒化壳聚糖软膏促浅Ⅱ度烫伤小鼠创面愈合的作用。方法:147只昆明种小鼠随机分为硒化壳聚糖软膏治疗组、基质治疗组和京万红治疗组,每组49只,采用将脱毛区置于70℃恒温水浴中6s的方法制成10%体表面积浅Ⅱ度烫伤模型,伤情经病理切片证实。各组创面分别用硒化壳聚糖软膏纱布(1ml/cm2)、基质纱布(1ml/cm2)、京万红纱布(1ml/cm2)覆盖包扎固定后放回笼中饲养,换药1次/天,观察愈合时间,于伤后12h,第1、3、5、7和9天,分别处死各组小鼠7只,检测含水量、羟脯氨酸、TNF、NO、ALT,另取7只做为正常对照。结果:①创面愈合时间:硒化壳聚糖软膏组为(12.9±2.9)天、基质组为(16.3±2.1)天、京万红组为(11.8±2.4)天,硒化壳聚糖软膏组比基质组明显缩短(P<0.05);②创面含水量:伤后第1、3、5天硒化壳聚糖软膏组[(86.3±3.5)%、(77.8±3.9)%、(72.3±2.7)%]创面含水量显著低于基质组[(92.8±3.2)%、(84.9±4.2)%、(77.2±2.8)%,(P<0.05)],伤后7～9天,各组均基本恢复到正常水平;③创面TNF-α水平:伤后12h至1天达高峰,随后逐渐下降,至伤后第5天仍高于正常对照组(P<0.05);伤后12h、1天、3天、5天,硒化壳聚糖软膏组和京万红组TNF水平明显低于基质组(P<0.05);④创面组织NO含量:伤后12h各烫伤组NO含量达高峰,随后下降,至伤后第9天仍高于正常对照组(P<0.05);伤后12h、1天,硒化壳聚糖软膏组和京万红组NO含量明显低于基质组(P<0.05);⑤创面羟脯氨酸含量:伤后5、7、9天硒化壳聚糖软膏组羟脯氨酸含量显著高于基质组和京万红组(P<0.05)。结论:硒化壳聚糖软膏能有效减少烫伤后早期创面组织NO和TNF-α释放,减少渗出和水肿,晚期通过增强创面胶原合成来促进创面愈合。

Effect of Selenium chitosan on wound healing in vivo

Objective To investigate the effects of Selenium chitosan cream on wound healing of superficial second degree burn in rats.Methods A total of 147Kunming rats were randomly and equally divided into Selenium chitosan cream group 、ground substance group and JingWanHong group.Superficial second degree burn covering 10% of total body surface area was produced by immersing in 70℃ water for 6 second.The wounds were respectively covered with Selenium chitosan cream(1ml/cm2),ground substance(1ml/cm2),and JingWanHong(1ml/cm2).The rats were caged separately,and the dressings were changed once daily.The rats wound healing time were observed in all the groups.7 rats in each group were killed and the biopsies of wound tissues were taken on 12 hours and days1,3,5,7,9 after burn for the determination of water contents、hydroxyproline,NO,TNF,blood ALT and Crea.Another 7 rats were selected as control.Results ①The time of wound healing was(12.9±2.9)days in Selenium chitosan cream group,(16.3±2.1)days in ground substance group,and(11.8±2.4)days in JingWanHong group respectively.The time for wound healing in Selenium chitosan cream group was significantly shorter than that in ground substance group(P<0.05).②The water content in the wound tissue in Selenium chitosan cream group on days 1,3,5 after burn[(86.3±3.5)%、(77.8±3.9)%、(72.3±2.7)%]was lower than those in ground substance group[(92.8±3.2)%、(84.9±4.2)%、(77.2±2.8)%,(P<0.05)].During 7-9 days after burn,all of them returned to the normal level.③The TNF-αlevel in wound tissue in each group reached the peak at 12-24h after burn,decreased thereafter,but it was still obviuosly higher than that of normal controls on 5 days after burn(P<0.05).The TNF-α level in wound tissue in Selenium chitosan cream group and JingWanHong group were markly lower than those in ground substance group at 12h and 1、3、5d after burn(P<0.05).④The NO content in wound tissue in each group reached the peak at 12h after burn,decreased thereafter,but it was still obviuosly higher than that of normal controls on 9 days after burn(P<0.05).The NO content in wound tissue in Selenium chitosan cream group and JingWanHong group were markly lower than those in ground substance group at 12h and 1d after burn(P<0.05).⑤The hydroxyproline content in the wound tissue in Selenium chitosan cream group on days 5,7,9 after burn was higher than that in ground substance group(P<0.05)and in JingWanHong group(P<0.05).Conclusion Selenium chitosan cream can accelerate wound healing by decreasing wound tissue NO and TNF-α release、enhancing collagen synthesis and lessening permeability and edema.