HPD-300型大孔吸附树脂分离纯化连翘中连翘酯苷A的工艺研究

目的优化HPD-300型大孔吸附树脂分离纯化连翘酯苷A的工艺条件。方法以连翘酯苷A含量为指标,采用高效液相法,考察D-101型,D-201型,HPD-100型,HPD-300型等8种大孔吸附树脂对连翘中连翘酯苷A吸附纯化工艺条件。结果 HPD-300型大孔吸附树脂具有最佳的吸附洗脱参数,其动态饱和吸附-洗脱量达3.57%。其吸附分离连翘酯苷A的工艺条件为:上样浓度为0.3g(生药)/ml,吸附流速为1BV/h,以5倍柱体积水洗脱,继以7倍柱体积50%乙醇洗脱。结论该技术适用于连翘中连翘酯苷A粗提取的工业化生产,连翘酯苷A纯度可达50%左右。

Purification of Forsythoside A in Forsythia Suspense by HPD-300 Macroporous Resin

Objective To optimize the best condition of Forsythoside A by HPD300 macroporous resin. Methods D-101,D-201,HPD-100,HPD-300…8 types of macroporus absorption resins were used to separate and purify the Forsythoside A from Forsythia suspense,with the content of Forsythoside A as parameter by HPLC. Results The HPD300 macroporous resin had optimum absorption and elution parameters in the following condition with its dynamic saturated absorption ratio up to 3.57% in the following condition:the sample concentration was 0.3 g· ml-1,1 BV·h-1 through macroporous resin column,used five times volume of water to wash first,following by seven times volume of 50% ethanol to wash and the elution velocity was 1BV·h-1. Conclusion Forsythoside A can be successfully purified in the above condition and be scale-up to industrialized production,more than 50% content are available.