Ras gene activation in malignant cells of human ovarian carcinoma peritoneal fluids |
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Authors: | DN Dokianakis MN Varras M Papaefthimiou J Apostolopoulou H Simiakaki E Diakomanolis DA Spandidos |
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Institution: | (1) Laboratory of Virology, Medical School, University of Crete, Heraklion, Crete, Greece;(2) Department of Clinical Cytology, Alexandra Hospital, Athens, Greece;(3) Department of Gynecological Oncology, Alexandra Hospital, Athens, Greece |
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Abstract: | In this study, we showed, for the first time, the pattern of point mutations at codon 12 of the K-ras, H-ras and N-ras genes, using polymerase chain reaction and restriction fragment length polymorphism analysis in 47 malignant cytologic specimens of ovarian adenocarcinoma peritoneal fluids. Forty-seven % of the samples were found to carry a point mutation at codon 12 of K-ras gene. Also, 21 cystadenoma peritoneal fluids were used as control specimens for the detection of ras mutations. Fourteen % of these samples were found to carry a point mutation at codon 12 of the K-ras gene. The prevalence of K-ras gene mutations were statistically correlated with FIGO and surgical stage of the malignant specimens. Our data demonstrates that the K-ras gene mutations are mainly affected (47%) in the malignant cells of the peritoneal washings or ascites of women with ovarian adenocarcinomas and may have value for the early diagnosis and monitoring of these neoplasms. |
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Keywords: | ras oncogenes ascites peritoneal washings ovarian neoplasms PCR |
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