五苓散对肾性高血压大鼠降压作用的实验研究

目的　观察五苓散提取液对肾性高血压大鼠的实验治疗效果及其对大鼠尿量和血清Na+ 、K+ 、Cl-浓度的影响。方法　建立改进G 2K1C二肾一夹高血压大鼠模型。大鼠随机分为 6组 :假手术组 ,模型组 ,五苓散高剂量组 (80 g/kg) ,五苓散中剂量组 (40g/kg) ,五苓散低剂量组 (2 0 g/kg) ,氢氯噻嗪组 (2 5mg/kg)。灌胃给药期间测量大鼠尿量 ,给药 30d后测定大鼠尾动脉压 ,并取血测定血清Na+ 、K+ 、Cl-浓度。结果　五苓散高、中、低剂量组均能降低肾性高血压大鼠的血压 (P <0 .0 5或P <0 .0 1) ,但仍高于假手术组 (P <0 .0 1) ,而与氢氯噻嗪组比较无显著差异 (P >0 .0 5 )。五苓散高、中、低剂量组对大鼠有显著利尿作用 (P <0 .0 5或P <0 .0 1) ,但作用较氢氯噻嗪缓和 (P <0 .0 5或P <0 .0 1)。五苓散高、中、低剂量组和氢氯噻嗪组对Na+ 、Cl-浓度均无影响 (P >0 .0 5 ) ,氢氯噻嗪组大鼠血K+ 浓度降低 ,与假手术组及高、中、低剂量组比较有显著差异 (P <0 .0 1)。结论　五苓散提取液对肾性高血压大鼠具有利尿、降压作用 ,且不造成电解质紊乱

Effect of Wuling Powder on rats with renal hypertension

OBJECTIVE: To observe the therapeutic effect of Wuling Powder extract on rats with renal hypertension and to evaluate the influence of it on the volume of urine and the concentrations of Na(+), K(+), Cl(-). METHODS: Reformed Gold-blatt hypertension rat model (G-2K1C) was established. The rats were divided into 6 groups as follows: sham-operation group; model group, Wuling Powder high dosage group (80 g/kg), Wuling Powder middle dosage group (40 g/kg), Wuling Powder low dosage group (20 g/kg), and hydrochlorothiazide (HCT) group (25 mg/kg). Urine volume of the rats was measured during the experiment. Tail arterial pressure and Na(+)], K(+)], Cl(-)] in serum of the rats were detected after 30 days of treatment. RESULTS: The blood pressure of the G-2K1C rats was decreased in the three Wuling Powder groups (P<0.05 or P<0.01), but higher than that of the false-operation group (P<0.01), and there was no difference between each of the Wuling Powder groups and the HCT group (P>0.05). Diuretic effect of the three dosages of Wuling Powder was weaker than that of the HCT (P<0.05 or P<0.01). The effects of the three dosages of Wuling Powder and HCT on Na(+)] and Cl(-)] in the serum were not obviously different (P>0.05), but K(+)] of the HCT group was significantly decreased compared with that of the false-operation group and the three Wuling Powder groups (P<0.01). CONCLUSION: Wuling Powder extract had satisfying therapeutic effects in increasing the discharge of urine, decreasing the blood pressure and keeping the balance of the serum electrolyte contents in rats with renal hypertension.