丙氨酰胺谷氨酰胺对烫伤大鼠的作用

目的研究添加丙氨酰谷氨酰胺(Ala-Gln)全胃肠外营养对烫伤大鼠的蛋白质代谢、肠粘膜形态学、创面肉芽组织的影响。方法将22只30%TBSAⅢ度烫伤SD大鼠行颈外静脉插管后，随机分为传统TPN组(传统组)和添加二肽TPN组(二伏组)，每组各11例，两组接受等热量(780kg

Effect of alanyl-glutamine supplementcd total parenteral nutrition on burned rats

Objective To study the effect ofalanyl-glutamine(Ala-Gln)supplemented total prenteral nutrition (TPN) on peotein metabolism,intestinal mucosal morphology,granulation tissues on wound in burned rats. Method Twenty-two SD rats that were placed a central venous catheter and subjccted to 30% TBSA Ⅲ° burn were divided into two groups equally:conventional TPN group (control group)and Ala-Gln supplemented TPN group (Ala-Gln group) and Ala-Gln supplemented TPN group (Ala-Gln group).All rats received isocaloric (780kJ.kg-1.d-1) and isonitrogenous (1.8g.kg-1.d-1,48% of nitrogen was supplied with Ala-Gln in Ala-Gln group) TPN for 7 days.Nitrogen balance and cumulated nitrogen balance were monitored.At the end of TPN,serum total protein (TP),albumin (ALB),prealbumin (PAB),transferrin (TF),Gln in muscle were monitored;Jcjunal mucosal morphology and granulation tissues on wound were observed. Results serum PAB,TF,Gln in muscle in Ala-Gln group were significantly higher than those in control group (P<0.01);Ala-Gln supplemented TPN significantly increased jejunal villus height,crypt depth,mucosal thickness and villous surface area compared with conventional TPN(P<0.01);The density of capillary vessels and fibroblast in the granulation tissues in Ala-Gln group were significantly highermore than those in control group (P<0.01). Conclusion Ala-Gln supplemented TPN in burned rats may promote protein anabolism,attenuate protein catabolism,maintain intestinal mucosal structure,promote wound healing.