| Heptanol预处理对细胞膜及线粒体Cx43参与的大鼠心肌细胞缺氧/复氧损伤的作用研究 |
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| 引用本文： | 李伟科,钟国强,何燕,陈立,邬江涛. Heptanol预处理对细胞膜及线粒体Cx43参与的大鼠心肌细胞缺氧/复氧损伤的作用研究[J]. 中国医药指南, 2011, 0(18): 5-6,18 |
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| 作者姓名： | 李伟科 钟国强 何燕 陈立 邬江涛 |
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| 作者单位： | 广西医科大学第一附属医院心血管研究所(广西医科大学研究生学院); |
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| 基金项目： | 2009年广西研究生教育创新计划项目(No2009105981002M212) |
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| 摘 要： | 目的通过对细胞膜及线粒体Cx43表达变化的研究,探讨不同浓度庚醇(Heptanol)预处理对大鼠心肌细胞缺氧/复氧损伤作用。方法将SD乳鼠培养的心肌细胞随机分为5组:正常对照组(control组),缺氧/复氧组(AR组),溶酶组(DMSO组),0.1mmol/L庚醇预处理组(0.1HT组),0.5mmol/L庚醇预处理组(0.5HT组),1.0mmol/L庚醇预处理组(1.0HT组),2.0mmol/L庚醇预处理组(2.0HT组)。除Control组外,H/R组,DMSO组以及各浓度heptonal预处理组均进行缺氧/复氧处理。检测Cx43mRNA水平,并提取心肌细胞线粒体蛋白,应用Western blot检测其含量。结果 AR组、0.1HT组和0.5HT组较control组的Cx43mRNA表达水平及线粒体Cx43含量明显减少,差异有统计学意义(P<0.01)。1.0 HT组和2.0HT组与AR组相比较,Cx43mRNA表达水平及线粒体Cx43含量明显增加,差异有统计学意义(P<0.01)。结论 heptanol预处理可以逆转I/R导致的细胞膜及线粒体Cx43的减少,但作用效果与浓度有关。线粒体Cx43可能参与了heptanol预处理的心肌保护作用。
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| 关 键 词： | 庚醇 缺氧/复氧 缝隙连接蛋白Cx43 |
Effects of Heptanol Precondictioning on Cx43 of Cardiac Muscle Sarcolemma and Mitochondria in Rat Model of Anoxia/Reoxygenation Injury |
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| LI Wei-ke,ZHONG Guo-qiang,HE Yan,CHEN Li,WU Jiang-tao. Effects of Heptanol Precondictioning on Cx43 of Cardiac Muscle Sarcolemma and Mitochondria in Rat Model of Anoxia/Reoxygenation Injury[J]. Guide of China Medicine, 2011, 0(18): 5-6,18 |
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| Authors: | LI Wei-ke ZHONG Guo-qiang HE Yan CHEN Li WU Jiang-tao |
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| Affiliation: | LI Wei-ke,ZHONG Guo-qiang,HE Yan,CHEN Li,WU Jiang-tao(Department of Cardiology,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China) |
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| Abstract: | Objective To investigate the effects of different concentration heptanol pre-condictioning on Connexin43 of cardiac muscle sarcolemma and mitochondria Cx43 in rat's myocardial cells model of anoxia/reoxygenation.Methods The myocardial cells which isolated from SD neonatal rats and cultured were divided randomly into 7 groups,namely: normal control group,anoxia/reoxygenation group,DMSO group,0.1mmol/LHT group,0.5mmol/LHT group,1.0mmol/LHT group,2.0mmol/LHT group.Each group was intervened appropriately before... |
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| Keywords: | Heptanol Anoxia/Reoxygenation Cx43-GJ |
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