安胃汤对慢性萎缩性胃炎模型大鼠EGF EGFR mRNA表达的影响

目的:探讨安胃汤对实验性慢性萎缩性胃炎大鼠模型的EGF、EGFR mRNA表达的影响。方法:采用甲基硝基亚硝基胍(MNNG)慢性萎缩性胃炎大鼠模型,48只清洁级健康雄性Wistar大鼠随机分为空白组、病理模型组、安胃汤组、胃复春对照组共4组。实时荧光定量PCR方法观察安胃汤对实验性慢性萎缩性胃炎大鼠模型胃黏膜EGF、EGFRmRNA表达的影响,对后者进行图像分析及统计学处理。结果:与病理模型组相比,安胃汤组和胃复春对照组均显著降低EGFmRNA/β-actin值(0.32±0.04 vs 0.64±0.08,0.42±0.05 vs 0.64±0.08,P<0.01),安胃汤组优于胃复春对照组(P<0.01)。与病理模型组相比,安胃汤组和胃复春对照组均显著降低EGFRmRNA/β-actin值(0.56±0.11vs 0.78±0.14,0.67±0.08 vs 0.78±0.14,P<0.01),安胃汤组优于胃复春对照组(P<0.05)。结论:安胃汤可能通过降低胃黏膜EGF、EGFR mRNA表达而起到治疗CAG作用。

Effect of Anwei Decoction on Expression of EGF, EGFR mRNA in Rats with Chronic Atrophic Gastritis

Objective： To explore the effect of Anwei Decoction （AD） on the expression of EGF, EGFR mRNAs in rats with chronic atrophic gastritis （CAG）. Methods： Forty eight healthy rats were randomly divided into 4 groups： normal control group, pathologic model group, Anwei Decoction group, and Weifuehun group. CAG was induced in rats with N-methy-N-nitro-N-ni- troguanidine （MNNG）. The effects of these medicines on the expressions of EGF, EGFR mRNAs in rats＇ gastric mueosas were determined by real-time polyrnerase chain reaction （real-time PCR）. Results： Compared with the pathologic model group, the expression of EGF mRNA was remarkably reduced in Anwei Decoction and Weifuchun groups （0. 32±0. 04 vs 0. 64±0. 08, 0. 42 ±0. 05 vs 0. 64±0. 08, P〈0. 01 ）. The expression level of EGFR mRNA was significantly lower in Anwei Decoction group than in Weifuehun group （P〈0.01）. Compared with the pathologic model group, the expression of EGFR mRNA was remarkably reduced in Anwei Decoction and Weifuchun groups （0. 56±0. 11 vs 0. 78±0. 14, 0. 67±0. 08 vs 0. 78±0. 14, P〈0.01）. The ex- pression level of EGFR mRNA was significantly lower in the Anwei Decoction group than in the Weifuchun group （ P〈0. 05 ）. Conclusion： Anwei Decoction may treat CAG by reducing the expression of EGF, EGFR mRNA in gastric mucosas.