Survivin在胆囊癌中的表达及其与p53相关性研究

目的检测胆囊癌中凋亡抑制因子Survivin的表达，研究其与胆囊癌f临床特征的关系，并探讨其与p53的相关性。方法采用免疫组织化学技术检测45例胆囊癌组织、20例胆囊息肉、20例胆囊炎及10例正常胆囊组织中Survivin和p53表达。结果45例胆囊癌组织中40例表达Survivin蛋白，34例表达p53蛋白，表达p53的病例中，31例表达Survivin阳性。而在胆囊息肉、胆囊炎及正常胆囊组织中不表达。且Survivin的表达与胆囊癌病人性别、年龄、肿瘤大小、病理分级、Nevin分期、淋巴结转移无关（P〉0．05），而p53与淋巴结转移密切相关（P〈0．01）。结论Survivin在胆囊癌中的高表达提示其可能与肿瘤的发生有关，Survivin和p53突变对凋亡抑制的协同作用在胆囊癌的发生发展过程中起重要作用。

Expression of survivin and its relation to p53 in gallbladder cancer

Objective To investigate the expression and its relation to p53 in gallbladder cancer. Methods The expression of survivin and p53 was determined with immunohistochemistry in samples from 45 cases of gallbladder, 20 chronic cholecystitis, 20 polypoid lesions of gallbladder (PLG) and 10 healthy individuals. Results Amongst the 45 cases of gallbladder cancer, 40 expressed survivin and 34 expressed p53. Of the 34 p53-positive cases, 31 were positive for expression of survivin. Survivin was not found in tissues of chronic cholecystitis, PLG and normal gallbladder. The expression of survivin in gallbladder cancer was not related to age, gender, tumor size, pathological grade, clinical stage and lymph node metastasis (P>0. 05) but that of p53 was associated with lymph node metastasis (P<0. 01). Conclusions Survivin might be correlated with the occurrence of gallbladder cancer. Joint action of survivin and p53 mutation on apoptosis inhibition plays an important role in the formation and metastasis of gallbladder cancer.