黏着斑激酶在结肠癌组织中的表达及意义

目的探讨黏着斑激酶（FAK）在结肠癌发生、发展中的作用。方法采用RT-PCR法检测30例新鲜结肠癌及与之相对应的癌旁组织的FAK mRNA表达；同时用Western印迹法检测20例新鲜结肠癌及相对应的癌旁组织FAK蛋白表达水平，调平每对组织FAK蛋白含量后再行FAK Tyr397磷酸化位点蛋白检测。结果30例结肠癌组织FAK mRNA阳性率为90．0％，其表达值为0．745±0．530，对应癌旁组织阳性率为43．3％，其表达值为0．241±0．131（P〈0．01）；20例结肠癌组织FAK蛋白阳性率为95．0％，表达值为0．482±0．150；对应癌旁组织阳性率为60．0％，表达值为0．269±0．015；P均〈0．01。20例结肠癌组织中FAK Tyr397磷酸化蛋白阳性率为90．0％（18／20），表达值为0．385±0．021；而对应癌旁组织阳性率为20％（4／20），表达值为0．110±0．005，P均〈0．01。结论FAK特别是FAK Tyr397磷酸化蛋白表达增加在结肠癌的发生、发展中可能起重要作用。

Expression and significance of focal adhesion kinase in colon carcinoma tissue

Objective To investigate the expression of focal adhesion kinase（FAK） and its significance in the pathogenesis and development of colon carcinoma. Methods Semi-quantitative RT-PCR method was used to detect FAK mRNA expression in colon carcinoma tissue and their corresponding para-cancer tissue of 30 patients. FAK （ including Phespho- FAK） protein expression were detected in the colon carcinoma tissue and in the para-cancer tissue of 20 patients by western bloting. Results The positive rate of FAK mRNA expression in the cancer tissue was significantly higher than that in the corresponding para-cancer tissues （90.0% VS 43.3% ）, with the level of 0.745± 0. 530 and 0.241 ± 0.131 （P 〈 0.01 ）. The positive rate of FAK protein expression in the cancer tissue was 95.0% with the level of 0.482 ± 0. 150, which in the corresponding para-cancer tissue was 60.0% and 0.269 ± 0.015 （ P 〈 0.01 ）. The positive rate of Tyr-397 FAK expression in the cancer tissue was 90.0% with the level of 0.385 ± 0.021, which in the corresponding para-cancer tissues was 25% and 0. 110 ±0.005 （ P 〈 0.01 ）. Conclusion The up-regulation of FAK expression（ especially Tyr-397 FAK protein） may play an important role in the tumorigenesis and progress of colon carcinoma.