| 摘 要： | AIM:γ-glutamyl transpeptidase(GGT)has been reportedas a virulence and colonizing factor of Helicobacter pylori(H pylori).This study examined the effect of GGT on thegrowth of H pylori.METHODS:Standard H pylori strain NCTC 11637 and 4dinical isolates with different levels of GGT activity as measuredby an enzymatic assay were used in this study.Growthinhibition and stimulation studies were carried out by culturingH pylori in brain heart infusion broth supplemented withspecific GGT inhibitor(L-serine sodium borate complex,SBC)or enhancer(glutathione together with glycyl-glycine),respectively.The growth profiles of H pylori were determinedbased on viable bacterial count at time interval.RESULTS:Growth was more profuse for H pylori isolateswith higher GGT activity than those present with lower GGTactivity.However,in the presence of SBC,growth of H pyloriwas retarded in a dose dependent manner(P=0.034).Incontrast,higher growth rate was observed when GGTactivity was enhanced in the presence of glutathione andglycyl-glycine.CONCLUSION:Higher GGT activity provides an advantageto the growth of H pylori in vitro.Inhibition of GGT activityby SBC resulted in growth retardation.The study showsthat GGT plays an important role on the growth of H pylori.
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