树突状细胞诱导的细胞毒T淋巴细胞抑制移植瘤细胞增殖

目的　研究树突状细胞（ Ｄ Ｃ）体外诱导的细胞免疫能否抑制裸鼠移植瘤肿瘤细胞增殖。方法　联合应用粒／巨细胞集落刺激因子（ Ｇ Ｍ  Ｃ Ｓ Ｆ）及白介素４（ Ｉ Ｌ４）直接从肝癌患者外周血中培养出 Ｄ Ｃ；以源于人肝癌细胞系 Ｈｅｐ Ｇ２ 细胞的肿瘤抗原粗提物刺激 Ｄ Ｃ； Ｄ Ｃ 激活同源的 Ｔ 淋巴细胞产生细胞 毒性 Ｔ 淋 巴细胞 （ Ｃ Ｔ Ｌ）； 建立 人肝癌 细胞 系 Ｈｅｐ Ｇ２ 裸鼠移植瘤模型； Ｃ Ｔ Ｌ 治疗人肝癌细胞系 Ｈｅｐ Ｇ２ 裸鼠移植瘤；检测移植瘤标本肿瘤细胞 Ｐ Ｃ Ｎ Ａ 表达水平。结果　 Ｄ Ｃ诱导的 Ｃ Ｔ Ｌ抑制 Ｈｅｐ Ｇ２ 裸鼠移植瘤细胞增殖从而抑制移植瘤生长。结论　经肿瘤抗原激活的 Ｄ Ｃ 作为一新概念上的抗肿瘤疫苗有可能在肿瘤的治疗中发挥重要作用。

IMMUNE RESPONSE INDUCED BY DENDRITIC CELLS INHIBITS PROLIFERATION OF IMPLANTED TUMOR CELLS IN NUDE MICE

Objective To study if the immune response in vivo induced by dendritic cells(DC) pulsed with tumor extract can inhibit the growth of implanted tumor in nude mice.Methods Granulocyte/macrophage colony stimulating factors and interleukin 4 were used to cultivate DC from peripheral blood of hepatocellular carcinoma patients.Tumor associated antigen (TAA) extracted from human liver cancer HepG2 cell line was used to activate the DC,which will initiate homogenic T lymphocyte to form cytotoxic T lymphocyte (CTL).HepG2 cells were transplanted to the nude mice and were treated with CTL.Expression of PCNA in tumor cells was evaluated.Results The DC from HCC patient pulsed with TAA from HepG2 tumor cells can initiate T lymphocyte immune response to inhibit the growth of implanted tumor in nude mice.Conclusions As a new concept of anti tumor vaccine,DC pulsed by TAA may play an important role in treating liver cancer in vivo.$$$$