酪氨酸激酶受体C在膀胱癌SCaBER细胞裸鼠种植瘤中的作用

【目的】通过酪氨酸激酶受体C(tyrosine receptor kinase,TrkC)siRNA干涉质粒下调膀胱癌细胞SCaBER裸鼠种植瘤中TrkC的表达,分析TrkC在膀胱癌中的作用。【方法】采用构建SCaBER细胞裸鼠种植瘤模型,以脂质体Lipofect Amine 2000为介质,采用瘤内注射的方法,用TrkCsiRNA干涉质粒实现下调SCaBER细胞中TrkC的表达,以空载体pSilencer作为阴性对照,干涉过程中记录裸鼠种植瘤的体积变化,绘制种植瘤生长曲线,干涉结束后处死裸鼠,剥除肿瘤,拍照,称重,并提取蛋白,western blotting检测干涉效果。【结果】Western blotting检测发现,构建的TrkCsiRNA干涉质粒顺利实现了在SCaBER细胞裸鼠种植瘤中对TrkC的表达的下调(F=7.46,P<0.05),种植瘤生长曲线表明下调TrkC的表达可以抑制SCaBER细胞在裸鼠活体内的增殖,实验组肿瘤肿瘤体积(F=11.50,P<0.05)和重量(F=19.32,P<0.01)明显低于对照组。【结论】下调TrkC表达可抑制膀胱癌细胞SCaBER在活体内增殖,TrkC可能参与膀胱癌发生发展过程。

Analyzing the function of tyrosine kinase c in bladder cancer cell-SCaBER xenograft in vivo

Objective]To analyze the function of tyrosine kinase receptor C （TrkC） in bladder cancer by down-regulating the TrkC expression level in SCaBER cell xenografts in vivo. Methods]SCaBER cells were injected into nude mouse to construct the xenografts model. Using LipofectAmineTM2000 reagent, the human bladder cancer cell line SCaBER was transfected with siRNA eukaryotic expression vectors pSilencer/TrkC and control plasmid pSilencer. Tumor volumes were recorded during the interference, and tumor growth curves were drawn based on the data. After the interference, the nude mice were sacrificed and the tumors were removed, photoed and weighed. Western blotting was used to testify the protein level in the transfected and parental SCaBer cells. Results] The results of western blotting indicated that TrkC expression levels in experimental group were lower than the eontrols（F = 7.46, P 〈 0.05）. After down-regulating the TrkC expression level of TrkC in SCaBER cell xenografts, we found that the tumor growth curves showed that the growth of SCaBER cell xenografts was inhibited in the experimental groups compared with the controls（F = 1 1.50, P 〈 0.05）. The weights of experimental group were also significantly lower than the eontrols（F = 19.32, P 〈 0.01）. Conclusions]Down-regulation of TrkC could inhibit the proliferation of SCaBER cell xenografts in vivo. TrkC might be correlated with carcinogensis and development of bladder cancer.