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| 滇重楼内生细菌分离及抑制人体病原菌活性菌株筛选 | |
| 引用本文： | 宣群,王芳,杨娟. 滇重楼内生细菌分离及抑制人体病原菌活性菌株筛选[J]. 昆明医学院学报, 2010, 31(3): 21-23 |
| 作者姓名： | 宣群 王芳 杨娟 |
| 作者单位： | 1. 昆明医学院微生物学暨免疫学教研室,云南,昆明,650500 2. 昆明医学院科学技术处,云南,昆明,,650500 3. 思茅师范高等专科学校生命科学系,云南,普洱,665000 |
| 基金项目： | 云南省教育厅自然科学基金资助项目(06Y043C) |
| 摘 要： | 目的分离滇重楼内生细菌,研究其抑制人体病原菌的能力.方法从表面消毒的滇重楼新鲜植株中分离内生细菌.在体外,用提取物抑制金黄色葡萄球菌、伤寒沙门菌、普通变形杆菌、痢疾杆菌、大肠杆菌及白色念珠菌生长.结果来自温室的植株分离到更多的内生细菌,植株地下部位中内生细菌比地上部位更多;约60%内生细菌对指示菌有一定抑制作用,其中LRB11抑菌效果较明显.结论由于内生细菌与宿主相互作用,故具抑菌作用的内生细菌可用于新药的开发或中药植物的生防. |
| 关 键 词： | 滇重楼 内生细菌 抑菌活性 |
Isolation and Screening of Endophytic Bacteria with Antimicrobial Activity from Paris Polyphylla Smith var.Yunnanensis |
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| XUAN Qun,WANG Fang,YANG Juan. Isolation and Screening of Endophytic Bacteria with Antimicrobial Activity from Paris Polyphylla Smith var.Yunnanensis[J]. Journal of Kunming Medical College, 2010, 31(3): 21-23 | |
| Authors: | XUAN Qun WANG Fang YANG Juan |
| Abstract: | Objective To isolate endophytic bacteria from Paris Polyphylla Smith var.yunnanensis,investigate the ability of these isolates to inhibit pathogenic microbes of human.Methods Endophytic bacteria were obtained from surface-sterilized fresh plant.In vitro,the extracts of endophytic bacteria were used to inhibit the growth of Staphylococcus aureus,Salmonella typhi,Proteus vulgaris,Shigella dysenteriae,E.coli and Candida albicans.Results Isolates were more from the plants culturing in greenhouse than that in fo... |
| Keywords: | Paris Polyphylla Smith var.yunnanensis Endophytic bacteria Antimicrobial activity |
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