小鼠骨髓基质细胞移植对急性辐射造血损伤的影响

目的：探讨小鼠骨髓基质细胞（marrow stromal cell,MSC）同种异体移植对接受全身大剂量辐射小鼠造血修复的影响。方法：在体外对小鼠骨髓基质细胞进行培养、扩增,并将第三代细胞通过尾静脉移植到同种接受全身大剂量射线照射的小鼠,测定移植后不同时期小鼠外周血白细胞（WBC）、红细胞（RBC）、血小板（PLT）、血红蛋白（HGB）值,观测各组脾集落形成单位（colony forming unit,CFU-S）,探讨同种异体骨髓基质细胞移植对急性辐射造血损伤修复的影响。结果：经骨髓基质细胞移植的小鼠,其WBC、RBC、PLT、HGB在移植后15-20 d较对照组明显增高（WBC、PLT、HGB：P〈0.01;RBC：P〈0.05）,移植组CFU-S明显高于对照组（P〈0.01）。结论：同种异体小鼠骨髓基质细胞移植,能促进小鼠造血干细胞在脾脏定居,增强造血干细胞形成集落的能力,并能显著提高外周血细胞数量,促进损伤后早期造血功能重建。

Effect of transplanted bone marrow stromal cells on irradiation injured in mice

Objective:To study the hemopoiesis effect of allogeneil graft of marrow stromal cell(MSC) on irradiation injured in mice.Methods:MSC from mice were cultured and proliferated in vitro,and then they were grafted into irradiated BALB/c mice via intravenous injection.After MSC transplantation were performed for various times,the levels of white blood cell(WBC),red blood cell(RBC),blood platelet(PLT),hemoglobin(HGB)and colony forming unit(CFU-S) were examined.Result:After the MSC transplantation were performed for 15 to 20 days,the levels of all of above indexes of the transplanted-group were significantly higher than those of the irradiate-group(P<0.05 to 0.01).Conclusion:Allogeneil graft of marrow stromal cell can induce hemopoietic stem cell to locate in the spleen and form unit in mice,increase significantly the peripheral blood cells and promote the early hemopoietic reconstitution after irradiation injury.