| 蛋白质组学技术鉴定帕金森病模型中的3种新蛋白质 |
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| 引用本文： | 张艳梅,马飞煜,邬伟,王秋艳,李兰根,胡林森. 蛋白质组学技术鉴定帕金森病模型中的3种新蛋白质[J]. 国际神经病学神经外科学杂志, 2008, 35(4) |
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| 作者姓名： | 张艳梅 马飞煜 邬伟 王秋艳 李兰根 胡林森 |
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| 作者单位： | 1. 吉林大学第一临床医学院神经内科,吉林省长春市
2. 内蒙古自治区医院,内蒙古呼和浩特市,010010 |
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| 摘 要： | 目的 利用蛋白质组学技术对MPP 诱导的PC12细胞帕金森病(PD)模型进行研究,以期发现PD发病机制中的新蛋白.方法 建立MPP 诱导的PC12细胞PD模型并提取细胞总蛋白,荧光差异凝胶电泳(D1GE)进行蛋白样品标记与分离,应用DeCyder软件分析蛋白质差异表达信息,运用MALDI-TOF质谱鉴定差异蛋白质.结果 质谱分析和数据库检索鉴定了3种可能同PD发病相关但在有关PD研究文献中尚未见报道的新蛋白质,他们分别足与线粒体功能相关的蛋白MPPs,具有分子伴侣活性的蛋白NAC和与免疫炎症相关的蛋白gc1qBP.结论 这些新发现蛋白可能与PD的发病机制密切相关.
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| 关 键 词： | PC12细胞 蛋白质组学 荧光差异凝胶电泳 |
Proteomic study found three new proteins in MPP~+-induced Parkinson''s disease model |
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| ZHANG Yan-Mei,Ma Fei-Yu,WU Wei,WANG Qiu-yan,LI Lan-gen,HU Lin-sen. Proteomic study found three new proteins in MPP~+-induced Parkinson''s disease model[J]. Journal of International Neurology and Neurosurgery, 2008, 35(4) |
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| Authors: | ZHANG Yan-Mei Ma Fei-Yu WU Wei WANG Qiu-yan LI Lan-gen HU Lin-sen |
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| Affiliation: | ZHANG Yan-Mei,MA Fei-Yu,WU Wei,WANG Qiu-yan,LI Lan-gen,HU Lin-sen.Department of Neurology,First Hospital of Jilin University,Changchun 130021,China |
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| Abstract: | Objective To find new proteins related to the pathogenesis of Parkinson's disease(PD),the proteomic technique was used to study the PC12 cells treated with MPP+.Methods We established the PD model of PC12 cell with MPP+,extracted the total proteins,isolated and labelled proteins with differential gel electrophoresis.Finally we identified the altered proteins with MALDI-TOF MS.Results Three proteins,NAC,MPPs and gC1qBP were identified by MALDI-TOF MS,which are possibly related to the pathogensis of PD and ha... |
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| Keywords: | MPP |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
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