| 离子对高效液相色谱法测定人血中二甲双胍含量 |
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| 引用本文： | 林观样,张秀华,张春红. 离子对高效液相色谱法测定人血中二甲双胍含量[J]. 中国现代应用药学, 2005, 22(3): 241-243 |
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| 作者姓名： | 林观样 张秀华 张春红 |
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| 作者单位： | 温州医学院附属第一医院,浙江,温州,325000 |
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| 摘 要： | 目的建立离子对-HPLC法测定人血中二甲双胍含量的方法.方法采用ODSC18色谱柱(4.6mm×250mm,5μm);以甲醇-磷酸二氢钾溶液(含0.03mol·mL-1庚烷磺酸钠,pH4.7)(4060)为流动相,流速1.0mL·min-1,紫外检测波长232nm,柱温37℃.结果离子对-HPLC法测定人血中二甲双胍的线性范围0.25～4μg·mL-1,相关系数0.9993.日内差异、日间差异均小于10%,绝对回收率大于80%.结论本法适用于二甲双胍血药浓度测定.
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| 关 键 词： | 离子对-HPLC 二甲双胍 含量测定 |
Ion pair HPLC assay of metformin hydrochloride in human plasma |
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| LIN Guan-yang,ZHANG Xiu-hu,ZHANG Chun-hong. Ion pair HPLC assay of metformin hydrochloride in human plasma[J]. The Chinese Journal of Modern Applied Pharmacy, 2005, 22(3): 241-243 |
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| Authors: | LIN Guan-yang ZHANG Xiu-hu ZHANG Chun-hong |
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| Abstract: | OBJECTIVE A HPLC method was established for the assaying of metformin in human plasma. METHODS The mosulfonate and adjusted to pH 4.7 by phosphoric acid) (40:60) ,the flow rate was 1.0mL/min, the detection was carried out with a than 10%. CONCLUSION The method is simple, rapid, and accurate, and it is suitable for the determination of metformin in the human plasma. |
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| Keywords: | ion pair HPLC metformin assay |
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