舒心益脉煎对气滞血瘀型大鼠动脉内膜损伤后细胞凋亡及基因蛋白产物的影响

探讨气滞血瘀型大鼠胸主动脉内膜剥脱后内膜增生中，舒心益脉煎对血管平滑肌细胞凋亡及相关基因Fas抗原和Fas配体的影响。将58只大鼠随机分为假且10只，舒心益脉煎组和对照组各24只。后两组大鼠行胸主动脉内膜剥脱术，舒心益脉煎组动物于术前7d至术后14d每日接受舒心益脉煎治疗。术后14d处死动物，用免疫组织化学法测定Fas抗原与Fas配体在血管中的表达，原位末端标记法测定凋亡细胞。术后14d血管新生内膜中有平滑肌细胞凋亡；舒心益脉煎显著增加新生内膜中Fas抗原与Fas配体的表达，增加血管平滑肌细胞凋亡，减少新生内膜面积。说明舒心益脉煎可能通过Fas系统调节动脉血管内皮损伤修复过程中血管平滑肌细胞凋亡，从而抑制新生内膜形成。

Effect of Shuxin Yimai Jian on the Apoptosis of Vascular Smooth Muscle Cells and Gene-protein Products after Injury of Thoracic Aorta Intima in Rats with Stagnancy of Qi and Blood Stasis

Aim To investigate the effect of Shuxin Yimai Jian (SXYMJ) on the apoptosis and the related gene Fas antigen and Fas ligand (FasL) of vascular balloon injury in thoracic aorta in rats with stagnancy of qi and blood stasis. Methods Fifty-eight rats were randomly divided into the sham group (n=10), SXYMJ group (n=24) and the control group (n=24). The thoracic aorta endothelium was denudated in the SXYMJ and control groups. SXYMJ was given from 7 days before operation to 14 days after operation in the SXYMJ group. Fas and FasL were detected by immunohistochemistry and the apoptotic cells were stained in situ by terminal deoxynucleotidyl transferase mediated-deoxyuridine triphosphate nick end labeling. Results Apoptotic cells could be detected in the smooth muscle cell (SMC) at the 14th day after operation. The expression of Fas and FasL in the neointima was increased by SXYMJ. The apoptosis of SMC was enhanced by SXYMJ, while the neointima area was attenuated. Conclusion SXYMJ probably regulates the apoptosis of SMC through Fas system and then inhibits the neointima hyperplasia.