Assessment of intensive care unit‐acquired weakness in young and old mice: An E. coli septic peritonitis model |
| |
| Authors: | Esther Witteveen MD Inge C.M. Hoogland MD Luuk Wieske MD PhD Nina C. Weber PhD Camiel Verhamme MD PhD Marcus J. Schultz MD PhD Ivo N. van Schaik MD PhD Janneke Horn MD PhD |
| |
| Affiliation: | 1. Department of Intensive Care Medicine, Academic Medical Center, Amsterdam, the Netherlands;2. Laboratory of Experimental Intensive Care and Anesthesiology (L·E·I·C·A), Academic Medical Center, Amsterdam, the Netherlands;3. Department of Neurology, Academic Medical Center, Amsterdam, the Netherlands;4. Center for Experimental Molecular Medicine, Academic Medical Center, Amsterdam, the Netherlands |
| |
| Abstract: | Introduction: There are few reports of in vivo muscle strength measurements in animal models of ICU‐acquired weakness (ICU‐AW). In this study we investigated whether the Escherichia coli (E. coli) septic peritonitis mouse model may serve as an ICU‐AW model using in vivo strength measurements and myosin/actin assays, and whether development of ICU‐AW is age‐dependent in this model. Methods: Young and old mice were injected intraperitoneally with E. coli and treated with ceftriaxone. Forelimb grip strength was measured at multiple time points, and the myosin/actin ratio in muscle was determined. Results: E. coli administration was not associated with grip strength decrease, neither in young nor in old mice. In old mice, the myosin/actin ratio was lower in E. coli mice at t = 48 h and higher at t = 72 h compared with controls. Conclusions: This E. coli septic peritonitis mouse model did not induce decreased grip strength. In its current form, it seems unsuitable as a model for ICU‐AW. Muscle Nerve 53 : 127–133, 2016 |
| |
| Keywords: | animal model critical illness myopathy critical illness polyneuropathy grip strength intensive care unit‐acquired weakness |
|
|
