| Toll样受体-4表达对树突状细胞表型及功能的影响 |
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| 引用本文： | 谢云青,栗世铀,刘施佳,黄丽洁,郑秋红. Toll样受体-4表达对树突状细胞表型及功能的影响[J]. 中国肿瘤生物治疗杂志, 2017, 24(9): 950-954. DOI: 10.3872/j.issn.1007-385x.2017.09.004 |
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| 作者姓名： | 谢云青 栗世铀 刘施佳 黄丽洁 郑秋红 |
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| 作者单位： | 1. 福建医科大学附属肿瘤医院福建省肿瘤医院福建省肿瘤生物治疗重点实验室,福建福州,350014;2. 中国科学院北京基因组研究所,北京,100020 |
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| 基金项目： | 国家临床重点专科建设项目资助；福建省科技计划项目资助（No.2017Y0022）；福建省自然基金项目资助（No.2016J01514） |
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| 摘 要： | 目的:探讨Toll样受体-4(TLR-4)表达对树突状细胞(DC)表型及功能的影响及其机制.方法:以构建的含有TLR-4全基因序列的pcDNA3.1质粒载体为模板,通过mMESSAGE mMACHINE T7 Kit体外转录获得TLR-4 mRNA,并采用脂质体法将其转染健康人外周血PBMC来源的DC,用流式细胞术检测鉴定转染后DC表面功能性分子的表达及DC体外诱导细胞毒性T淋巴细胞(CTL)分泌细胞因子的能力.结果:成功构建人TLR-4-pcDNA3.1质粒载体;体外扩增成功人TLR-4和EGFP mRNA片段.TLR-4 mRNA转染后的DC表面趋化因子CCR7及功能性分子HLA-DR的表达显著高于control mRNA转染DC、转染前成熟DC[CCR-7:(42.4±4.93)％ vs (20.1±3.09)％、(17.1±4.33)％,P＜0.05;HLA-DR:(62.1±7.23)％ vs(17.7±6.01)％、(25.8±4.16)％,P＜0.05],同时转染后的DC体外诱导CTL分泌IFN-γ细胞因子的能力强于control mRNA-DC、空载转染DC诱导的CTL及加入DC前CTL[(66.5±3.58)％ vs (41.1±4.27)％、(37.9±2.96)％、(3.2±2.03)％,P＜0.05].结论:TLR-4 mRNA转染DC可显著增强DC的功能,提高了DC抗原提呈及诱导CTL的能力,为增强DC疫苗抗肿瘤效应提供实验依据.
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| 关 键 词： | 树突状细胞 细胞毒性T淋巴细胞 Toll样受体-4 抗原提呈 免疫应答 |
| 收稿时间： | 2017-04-10 |
| 修稿时间： | 2017-06-15 |
Effect of TLR-4 on phenotypes and function of dendritic cells |
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| XIE Yunqing,LI Shiyou,LIU Shiji,HUANG Lijie and ZHENG Qiuhong. Effect of TLR-4 on phenotypes and function of dendritic cells[J]. Chinses Journal of Cancer Biotherapy, 2017, 24(9): 950-954. DOI: 10.3872/j.issn.1007-385x.2017.09.004 |
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| Authors: | XIE Yunqing LI Shiyou LIU Shiji HUANG Lijie ZHENG Qiuhong |
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| Affiliation: | 1. Fujian Provincial Key Laboratory of Tumor Biotherapy, Affiliated Tumor Hospital of Fujian Medical University and Fujian Cancer Hospital, Fuzhou 350014, Fujian, China; 2. Beijing Institute of Genomics, ChineseAcademy of Sciences, Beijing 100020, China,1. Fujian Provincial Key Laboratory of Tumor Biotherapy, Affiliated Tumor Hospital of Fujian Medical University and Fujian Cancer Hospital, Fuzhou 350014, Fujian, China; 2. Beijing Institute of Genomics, ChineseAcademy of Sciences, Beijing 100020, China,1. Fujian Provincial Key Laboratory of Tumor Biotherapy, Affiliated Tumor Hospital of Fujian Medical University and Fujian Cancer Hospital, Fuzhou 350014, Fujian, China; 2. Beijing Institute of Genomics, ChineseAcademy of Sciences, Beijing 100020, China,1. Fujian Provincial Key Laboratory of Tumor Biotherapy, Affiliated Tumor Hospital of Fujian Medical University and Fujian Cancer Hospital, Fuzhou 350014, Fujian, China; 2. Beijing Institute of Genomics, ChineseAcademy of Sciences, Beijing 100020, China and 1. Fujian Provincial Key Laboratory of Tumor Biotherapy, Affiliated Tumor Hospital of Fujian Medical University and Fujian Cancer Hospital, Fuzhou 350014, Fujian, China; 2. Beijing Institute of Genomics, ChineseAcademy of Sciences, Beijing 100020, China |
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| Abstract: | |
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| Keywords: | dendritic cell cytotoxic T lymphocyte(CTL) Toll-like receptor-4(TLR4) antigen presenting immune response |
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