成都地区成人急性呼吸道感染病例中副流感病毒检出情况分析

目的 了解成都地区成人急性呼吸道感染病例中副流感病毒（human parainfluenza virus,HPIV）感染状况,探索其流行特点并进行基因分析。方法 对2014年3月至2016年6月从1 039例成人急性呼吸道感染者痰液标本中提取的病毒核酸,采用逆转录-聚合酶链反应（RT-PCR）检测HPIV病毒表面糖蛋白——血凝素（HA）DNA,并从检出样本中随机选取12例阳性标本进行HA基因测序, 通过序列的比对分析同源性及变异情况。结果 在1 039份痰液标本中,HPIV阳性有110例,检出率为10.6%。其中 8 例HPIV-1 型、22例HPIV-2型、46例HPIV-3型,34例HPIV-4型;不同基因型的检出率在季节和年龄上有一定波动。随机选取的12例阳性标本中,亚型阳性标本之间HA基因核苷酸序列均没有明显差异,与参考序列相比差异小,与不同地域参考株序列相比,本次测定的毒拌与中国其他省市参考株之间的核苷酸遗传距离最小。结论 成都地区成人急性呼吸道感染者痰液标本中HPIV检出率较高,不同基因型均有检出并且以HPIV-3型为主。

Detection and Analysis of Human Parainfluenza Virus Infection in Hospitalized Adults with Acute Respiratory Tract Infections

ObjectiveTo investigate the prevalence and gene characteristics of different groups of human parainfluenza virus (HPIV) infection in hospitalized adults with acute respiratory tract infections (ARI). MethodsRT-PCR was used to detect HPIV hemagglutinin (HA) DNA, which was extracted from sputum samples of 1 039 adult patients with ARI from March, 2014 to June, 2016. The HA gene amplified from randomly selected positive samples were sequenced to analyze the homology and variation. Results10.6% (110/1 039) of these samples were positive for HPIV, including 8 cases of HPIV-1, 22 cases of HPIV-2, 46 cases of HPIV-3 and 34 cases of HPIV-4. Detectable rate varied among different groups of HPIV according to seasons of the year and ages of patients. No significant differences were found between the positive samples and the reference sequences. Compared with different reference strains of different regions, the genetic distance of nucleotide is the smallest between the strains tested in this study and the reference strains of other provinces and cities in China. ConclusionIn Chengdu region, HPIV virus is highly detected in ARI, all subtypes were detected with HPIV-3 being the main subtype.