蜂胶黄酮 pinobanksin-3-acetate对胃癌SGC-7901细胞增殖和凋亡的影响及机制

目的]探讨蜂胶黄酮pinobanksin-3-acetate对体外培养的胃癌SGC-7901细胞增殖、凋亡及部分基因表达的影响.方法]采用四甲基偶氮唑盐(MTr)显色法检测不同浓度PB3A作用不同时间对SGC-7901细胞生长所产生的影响,计算生长抑制率和IC50值;倒置显微镜观察PB3A干预后细胞的形态变化;Annexin V-FITC/PI双染色、流式细胞仪检测细胞凋亡率;Western blot法检测PB3A(40、80μg/ml)干预24h后SGC-7901细胞FOS、GEM、RGS2、GADD45G及HSPA6的蛋白表达水平,利用Spearman进行候选基因相关性分析.结果]PB3A可明显抑制SGC-7901细胞的增殖(P＜0.05),且抑制作用呈时间和剂量依赖性.随PB3A剂量的增多,SGC-7901细胞的凋亡率渐增,细胞的早期凋亡率从25.6％提高到50.2％.通过40μg/ml和80μg/ml PB3A干预胃癌SGC-7901细胞24h后与对照组相比,高浓度组GADD45G、HSPA6、GEM、RGSR及FOS蛋白表达有极显著性差异(P＜0.01),而低浓度组GADD45G、GEM和HSPA6蛋白表达有显著性差异(P＜0.05).结论]PB3A在体外可明显抑制胃癌SGC-7901细胞增殖并诱导凋亡的作用,并呈剂量依赖性变化趋势.机制可能与其诱导GEM、RGSR、FOS及HSPA6蛋白的上调表达有关,以上基因可能相互协同导致肿瘤细胞增殖信号通路的抑制并促进胃癌细胞的凋亡.

Effect of Propolis Flavonoid Pinobanksin-3-acetate on Proliferation,Apoptosis of Gastric Cancer SGC-7901 Cells and Related Mechanism

Purpose] To investigate the effects of propolis flavonoid pinobanksin-3-acetate(PB3A) on proliferation,morphology and apoptosis in human gastric cancer cell line SGC-7901 and the possible mechanism.Methods] Human gastric cancer AGC-7901 cells were treated with PB3A at concentration of 10,20,40 or 80μg/ml for 24,48 or 72h.The proliferation of SGC-7901 cells was analyzed by the MTT assay;the morphological changes of SGC-7901 cells were observed by the inverted microscopy;cell apoptosis was determined by FCM with Annexin V-FITC/PI double labeling;the expression of FOS,GEM,RGS2,GADD45G and HSPA6 proteins in SGC-7901 cells were detected by Western blot.Results] PB3A inhibited SGC-7901 cell proliferations in a time-and dose-dependent manner (P＜0.01).The FCM analysis showed that the PB3A significantly increased the apoptosis of SGC-7901 cells in a dose-dependent manner.Compared with control group,the expression of FOS,GEM,RGS2,GADD45G and HSPA6 was significantly increased in SGC-7901 cells treated with 80μg/ml PB3A for 24h (P＜0.01);while the expression of GADD45G,GEM and HSPA6 was increased in SGC-7901 cells treated with 40μg/ml PB3A(P＜0.05).Conclusion] PB3A can inhibit the proliferation of SGC-7901 cells by inducing apoptosis through up-regulation of FOS,GEM,RGS2,GADD45G and HSPA6 expression.