Neurotrophic Actions of Bone Marrow Stromal Cells on Primary Culture of Dorsal Root Ganglion Tissues and Neurons |
| |
Authors: | Yun Gu Jie Wang Fei Ding Nan Hu Yaxian Wang Xiaosong Gu |
| |
Institution: | (1) Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu, 226001, People’s Republic of China; |
| |
Abstract: | Application of adult bone marrow stromal cells (BMSCs) provides therapeutic benefits to the treatment of neurological insults.
The aim of this study was to explore the potential of nonhematopoietic BMSCs to produce soluble factors and stimulate signaling
pathways in neurons that mediate trophic effects. A combination of enzyme-linked immunosorbent assay and two-dimensional gel
electrophoresis coupled with mass spectrometry showed that the BMSCs released into the culture medium an array of soluble
factors such as nerve growth factor, brain-derived neurotrophic factor, basic fibroblast growth factor, and ciliary neurotrophic
factor, which have been shown to exhibit potent neurotrophic effects on neural cells. Immunochemistry, cell viability assay,
and quantitative real-time RT-PCR collectively showed that neurite outgrowth and neurogenesis in cultured rat dorsal root
ganglion (DRG) explants and neurons were enhanced after they were cocultured with rat BMSCs. Western blot analysis revealed
that BMSC-conditioned medium activated phosphorylation of mitogen-activated protein kinase/extracellular signal-regulated
protein kinase and/or phosphoinositide 3-kinase/serine/threonine kinase (PI3K/Akt) in primary culture of rat DRG neurons,
which suggested that BMSCs trigger endogenous survival signaling pathways in neurons through their secreted soluble factors.
Our data help to elucidate the mechanisms by which BMSCs function as a cell therapy agent in peripheral nerve regeneration. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|