蚕丝蛋白材料对鼠胚表皮细胞毒性的实验研究

目的研究不同蚕丝蛋白材料对鼠胚表皮细袍的毒性及其影响细胞增殖的因素。方法采用组织块培养法体外原代培养鼠胚表皮细胞，用直接接触法和浸提液法体外培养鼠胚表皮细胞，MTT比色法检测其在不同蚕丝蛋白材料上的增殖活力和细胞相对增殖率，进行毒性分析；并用活细胞计数法观察不同蚕丝蛋白材料对鼠胚表皮细胞生长的影响。结果鼠胚表皮细胞在1、2、4、5、7、8、10号各型蚕丝蛋白材料上生长，其增殖活力与细胞对照组相当（P〉0.05）；在3、6、9号化学交联或HY-823交联型蚕丝蛋白材料上生长，其增殖活力均低于细胞对照组（P〈0．05）；而在11号蚕丝蛋白材料其增殖活力显著低于细胞对照组（P〈0．01）。1、7号丝胶材料细胞毒性分级为0级（无毒），2、3、4、5、6、8、9、10号各型蚕丝蛋白材料细胞毒性分级为1级（轻微毒），而11号蚕丝蛋白材料细胞毒性分级为2级（中度毒）。活细胞计数结果与MTT检测结果基本相一致。结论除11号蚕丝蛋白材料外，1～10号各型蚕丝蛋白材料无明显细胞毒性，能支持鼠胚表皮细胞正常生长，具有良好的细胞相容性。

Study on Cytotoxity of Silk Protein Materials to Rat Embryo Epithelial Cells

Objective Toinvestigate cytotoxity of silk protein materials and their effect on prolif-eration of rat embryoepithelial cells.Methods Rat embryoepithelial cells were cultivated pri marilyinvitro bytissue culture and directly onsilk protein materials or extractions of the materials.The activityof cell proliferationandrelative growthrate(RGR) were assessed by MTTcolori metric assay.Thency-totoxity of the silk protein materials was analysed.The effect of silk protein materials on growth ofcells was observed by viable cell counts.Results The activity of cell proliferation on NO.1,2,4,5,7,8,10 silk protein materials was not lessthanthat inthe control group.The activity of cell proliferationon NO.3,6,9 silk protein materials waslower thanthat inthe control group.Whilethe activity of cellproliferation on NO.11 silk protein material obviouslylower thanthat inthe control group.The cyto-toxity rank of NO.1,7 sericin materials was 0.The cytotoxity rank of NO.2,3,4,5,6,8,9,10 silkprotein materials was 1.The cytotoxity rank of NO.11 silk protein material was 2.The result of vi-able cell counts was compatible to MTTcolori metric assay.Conclusion Except from NO.11 silk pro-tein material,NO.1 ~10 silk protein materials do not have obvious cytotoxity to rat embryo epithelialcells and are able tosustain normal growth of cells.The silk protein materials have a good cytocompat-ibility with rat embryo epithelial cells.